Effect of Probiotics Containing Yogurts on the Composition of Biofilms in Patients Under Orthodontic Treatment

Federal University of Pelotas

Status and phase: Completed; Phase 3; Phase 2

Conditions: Dental Caries; Oral Health

Treatments: Dietary Supplement: Placebo yogurt; Dietary Supplement: Yogurt containing probiotics

Study type: Interventional

Funder types: Other

Identifiers

NCT01657539

UFPEL-PPGO0013

Details and patient eligibility

About

Aim: To assess in vivo if the intake of yogurt containing Bifidobacterium animalis ssp. lactis DN- 1173010 probiotic for a period of 2 weeks affects the salivary levels or biofilms of mutans streptococci and the lactobacilli of patients undergoing orthodontic treatment. Methods: A crossover, double-blind, randomized and placebo controlled clinical trial will be performed with 26 volunteers during 4 periods. During periods 2 and 4 (2 weeks each) the volunteers will have a daily ingestion of yogurt containing probiotic or of control yogurt which did not contain probiotic. Periods 1 and 3 were run-in and wash-out periods of 1 and 4 weeks, respectively. Experimental samples of saliva and biofilm will be collected from each participant at the end of each phase. Mutans streptococci, lactobacilli, total aciduric microorganisms, Candida spp. and total cultivable microorganisms will be counted. The values before and after the treatment will be compared with Wilcoxon's test (p<0.05).

Enrollment: 30 patients

Sex: All

Ages: 14 to 30 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• excellent oral health
• patients under bimaxillary orthodontic treatment

Exclusion criteria

• any chronic disease
• antibiotics and/or antimicrobial use in the previous 3 weeks
• topic fluoride or chlorhexidine use in the previous 3 weeks
• presence of cavitated or active carious lesions
• patients with intolerance to lactose.

Trial design

Primary purpose: Prevention

Allocation: Randomized

Interventional model: Crossover Assignment

Masking: Quadruple Blind

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30 participants in 2 patient groups, including a placebo group

Probiotics

Experimental group

Description:

Group of patients using yogurt containing probiotics during the experimental phase of the study.

Treatment:

Dietary Supplement: Yogurt containing probiotics

Control Yogurt

Placebo Comparator group

Description:

Group of patients that will use a placebo yogurt for providing comparison with the experimental group.

Treatment:

Dietary Supplement: Placebo yogurt