Effect of Quercetin on Green Tea Polyphenol Uptake in Prostate Tissue From Patients With Prostate Cancer Undergoing Surgery

Jonsson Comprehensive Cancer Center

Status and phase: Completed; Phase 1

Conditions: Stage IV Prostate Cancer; Adenocarcinoma of the Prostate; Stage I Prostate Cancer; Stage IIA Prostate Cancer; Stage III Prostate Cancer; Recurrent Prostate Cancer; Stage IIB Prostate Cancer

Treatments: Procedure: therapeutic conventional surgery; Other: placebo; Dietary Supplement: green tea extract; Drug: quercetin; Other: laboratory biomarker analysis

Study type: Interventional

Funder types: Other; NIH

Identifiers

NCT01912820

R03CA171583 (U.S. NIH Grant/Contract)

12-000886

NCI-2013-01153 (Registry Identifier)

Details and patient eligibility

About

This randomized pilot phase I trial will evaluate if quercetin enhances the uptake of green tea polyphenols in the prostate tissue of men taking green tea extract and undergoing radical prostatectomy. Side effects of green tea extract and quercetin in combination with green tea extract will also be evaluated. In preclinical studies, green tea polyphenols have anticancer and cancer preventative effects in a number of malignancies. Likewise, in preclinical studies quercetin was found to enhance the anticancer effects of green tea. This trial is designed to translate these findings forward in a short-term human intervention trial.

Full description

PRIMARY OBJECTIVES:

I. To investigate the prostate tissue concentration of epigallocatechin gallate (EGCG), epicatechin gallate (ECG) and quercetin and their methylated metabolites in men scheduled for prostatectomy and consuming green tea (GT)extract (N=15) or GTextract with quercetin (N=15) for 3 weeks prior to prostatectomy.

II. To determine the concentration of EGCG, ECG, epigallocatechin (EGC), epicatechin (EC) and quercetin and the methylated metabolites in plasma collected before (T=0) and two hours (T=2) after the intake of the morning dose of GTextract and placebo or GTextract with quercetin during the third week of intervention.

III. To determine the effect of GTextract/quercetin intervention on reducing the enzyme activity and protein and gene expression of catechol-O-methyltransferase (COMT) and deoxyribonucleic (DNA) (cytosine-5) methyltransferase 1 (DNMT1) as well as protein and gene expression of multidrug resistance transport protein 1 (MRP1) in prostate tissue collected in specific aim 1 and COMT activity in erythrocytes from blood collected at baseline and week 3.

IV. To determine the inter-individual variation in genotype of COMT in buffy coat DNA.

OUTLINE: Patients are randomized to 1 of 2 treatment arms.

ARM I: Patients receive GT extract orally (PO) twice daily (BID) and quercetin PO BID for 3-6 weeks before undergoing prostatectomy.

ARM II: Patients receive GT extract PO BID and placebo PO BID for 3-6 weeks before undergoing prostatectomy.

Enrollment: 32 patients

Sex: Male

Ages: 40 to 75 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Subjects consent to participate in the trial
• The subject has a diagnosis of adenocarcinoma of the prostate
• The subject is scheduled to undergo radical prostatectomy
• The subject agrees to stop consuming tea or tea-containing products and quercetin supplements throughout the entire intervention period except for the green tea extract and quercetin provided during study intervention

Exclusion criteria

• History of hepatitis or liver dysfunction
• Ongoing alcohol abuse
• Significant medical or psychiatric conditions that would make the patient a poor protocol candidate
• Prior sensitivity or allergic reaction to tea, tea products or tea and quercetin supplements
• Allergies to multiple food items or nutritional supplements
• Taking luteinizing hormone-releasing hormone (LHRH) agonists, androgen receptor blocking agents, finasteride, or has undergone bilateral orchiectomy

Trial design

Primary purpose: Prevention

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: Double Blind

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32 participants in 2 patient groups, including a placebo group

Arm I (quercetin, green tea extract)

Experimental group

Description:

Patients receive GT extract PO BID and quercetin PO BID for 3-6 weeks before undergoing prostatectomy.

Treatment:

Other: laboratory biomarker analysis

Drug: quercetin

Procedure: therapeutic conventional surgery

Dietary Supplement: green tea extract

Arm II (GT extract, placebo)

Placebo Comparator group

Description:

Patients receive GT extract PO BID and placebo PO BID for 3-6 weeks before undergoing prostatectomy.

Treatment:

Other: laboratory biomarker analysis

Procedure: therapeutic conventional surgery

Dietary Supplement: green tea extract

Other: placebo