Effect of Smoking on the Expression of Gelatinases in Chronic Periodontitis

Baskent University

Status: Completed

Conditions: Periodontitis; Smoking

Treatments: Other: no intervention

Study type: Observational

Funder types: Other

Identifiers

NCT03426241

BaskentU2

Details and patient eligibility

About

This study comparatively evaluate the expressions of MMP-2 and MMP-9 in smoker vs non-smoker chronic periodontitis patients as well as periodontally healthy subjects. the hypothesis of this study was that smoking may interfere with periodontal disease by effecting MMP-2 and MMP-9 expressions.

Full description

Smokers have greater risk for developing periodontal disease and smoking is highly correlated with greater marginal bone loss. Matrix metalloproteinases (MMP) play a major role in periodontal tissue destruction. MMP-2 and MMP-9, called gelatinases, are one of the major MMPs that plays important roles in periodontitis. To date, several studies showed the increased levels of gelatinases in periodontal disease and only a limited number of study investigated the effect of smoking on gelatinases. However, no previous study has directly investigated the possible relationship between smoking and gingival tissue gelatinases expressions (MMP-2 and MMP-9) in chronic periodontitis (CP) patients relative to periodontally healthy subjects.

Therefore this study is conducted to comparatively evaluate the expressions of MMP-2 and MMP-9 in smoker vs non-smoker chronic periodontitis patients as well as periodontally healthy subjects. the of this study hypothesis was that smoking may interfere with periodontal disease by effecting MMP-2 and MMP-9 expressions.

Enrollment: 80 patients

Sex: All

Ages: 16 to 62 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• A total of 80 subjects (35 females, 45 males, with an age range of 16-62 years) were included in this study from those patients seeking dental treatment at the Baskent University School of Dentistry between February 2010 and December 2012.

Exclusion criteria

• Patients with systemic disease;
• pregnant and/or lactating women;
• patients taking any medications that may influence the study.

Trial design

80 participants in 4 patient groups

smoking chronic periodontitis

Treatment:

Other: no intervention

non-smoking periodontitis

Treatment:

Other: no intervention

smoking healthy

Treatment:

Other: no intervention

non-smoking healthy

Treatment:

Other: no intervention