Effect of Vegetable and Fruit Enzyme Supplementation on Muscle Activation, Aerobic Capacity, and Blood Biochemical Parameters After High-Intensity Interval Exercise: Feasibility Study of Exercise Design With Exergaming

Shu-Cheng Lin

Status: Enrolling

Conditions: Overtraining Syndrome

Treatments: Diagnostic Test: Training Impulse; Diagnostic Test: Blood Lactate Test; Diagnostic Test: Exergaming HIIE Test; Dietary Supplement: Supplementation Protocol

Study type: Interventional

Funder types: Other

Identifiers

NCT06466408

NCKU HREC-E-112-419-2

Details and patient eligibility

About

This study explores the effectiveness of fruit and vegetable enzyme supplementation in reducing fatigue and enhancing aerobic capacity in elderly individuals after engaging in high-intensity interval exercise (HIIE) through exergaming. Sixteen elderly female participants were divided into two groups based on pre-test lactate levels: an enzyme group and a placebo group, to ensure comparability. They performed HIIE using Nintendo Switch Ring Fit Adventure, completing eight sets of 20-second high-effort exercises with 30-second rest intervals, totaling 370 seconds of activity. The primary metrics evaluated were blood lactate levels, heart rate (HR), rating of perceived exertion (RPE), and training impulse (TRIMP). Participants in the enzyme group received a fruit and vegetable enzyme supplement in a 30 c.c. dose twice daily for 14 days. The study aimed to determine if enzyme supplementation could effectively mitigate the physiological stress of HIIE and improve exercise outcomes in the elderly.

Enrollment: 30 estimated patients

Sex: Female

Ages: 65+ years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

1. Aged 65 and above
2. healthy elderly participants
3. regular exercise habit (3 times per week within the past year)
4. Those who can move their upper and lower limbs normally

Exclusion criteria

1. no history of upper limb skeletal muscle injury or major injury
2. No heart-related diseases such as high blood pressure
3. No related chronic diseases, such as diabetes

Trial design

Primary purpose: Health Services Research

Allocation: Non-Randomized

Interventional model: Parallel Assignment

Masking: Single Blind

---

30 participants in 2 patient groups, including a placebo group

enzyme group

Experimental group

Description:

enzyme group consumed 30 mL of vegetable and fruit enzymes (The contents include needle-leaf cherries, cherries, apples, cranberries, blackberries, black currants, blueberries, beets, broccoli, cabbage, carrots, Concord grapes, cranberries, elderberries, kale, oranges, peaches, papayas, parsley, pineapples, raspberries, red currants, spinach, and tomatoes, etc.)(Enzyme Village, Chiayi, Taiwan) mixed with 150 mL of water twice a day (at breakfast and dinner) for 14 consecutive days.

Treatment:

Dietary Supplement: Supplementation Protocol

Diagnostic Test: Exergaming HIIE Test

Diagnostic Test: Blood Lactate Test

Diagnostic Test: Training Impulse

placebo group

Placebo Comparator group

Description:

The placebo group followed the same protocol but consumed malt syrup (Amazon, USA) mixed with 150 mL of water twice a day (at breakfast and dinner) for 14 consecutive days.

Treatment:

Dietary Supplement: Supplementation Protocol

Diagnostic Test: Exergaming HIIE Test

Diagnostic Test: Blood Lactate Test

Diagnostic Test: Training Impulse