Effect of Vitamin B12 and n-3 Polyunsaturated Fatty Acids on Plasma Homocysteine

Zhejiang University

Status: Unknown

Conditions: Healthy

Treatments: Dietary Supplement: vitaminB12; Dietary Supplement: Fish oil

Study type: Interventional

Funder types: Other

Identifiers

NCT01762072

No.30972464

Details and patient eligibility

About

Vitamin B12and n-3 polyunsaturated fatty acids (PUFA) decrease blood homocysteine (Hcy) concentration. However, the combined effect of these nutrients on Hcy and ferritin, and C-reactive protein is limited and inconclusive. The objective was to examine the synergistic effect of vitamin B12 in combination of n-3 PUFA on plasma Hcy, ferritin, and other biochemical markers. In a randomized controlled trial, thirty eligible subjects were randomly divided into three groups, and assigned to receive 1000 μg of vitamin B12, 2g fish oil, or 1000 μg vitamin B12 and 2g fish oil respectively for 8 weeks. Plasma phospholipid (PL) fatty acids and biochemical markers were determined. we hypothesize that combination of vitamin B12 and fish oil have synergistic effect on lowering plasma concentrations of Hcy and ferritin.

Enrollment: 30 estimated patients

Sex: All

Ages: 20 to 28 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• reliability, regular eating habits, normal weight, nondrinking, and nonsmoking status

Exclusion criteria

• None of the selected subjects used any vitamins or dietary supplements and was taking no medication for at least 8 weeks before the start of and during the entire experimental period

Trial design

Primary purpose: Prevention

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: Single Blind

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30 participants in 3 patient groups, including a placebo group

vitaminB12

Placebo Comparator group

Description:

VitB12 group (VitB12, n=10) receives 8 weeks of treatment with daily oral doses of 1000 μg of vitamin B12 (one capsule)

Treatment:

Dietary Supplement: vitaminB12

Fish oil

Experimental group

Description:

Fish oil group (FO, n=10)receives 8 weeks of treatment with daily oral doses of 2g of fish oil in the form of two capsules of fish oil) .

Treatment:

Dietary Supplement: Fish oil

Fish oil+vitaminB12

Experimental group

Description:

VitB12+Fish oil group (VitB12+FO, n=10) receives 8 weeks of treatment with daily oral doses of a combination of 1000 μg of vitamin B12 and 2g of fish oil

Treatment:

Dietary Supplement: Fish oil

Dietary Supplement: vitaminB12