Effect of Vitamin D Deficiency on the Structure of Titanium Platelet-Rich Fibrin (T-Prf)

Abant Izzet Baysal University

Status: Completed

Conditions: Vitamin D Deficiency; PRF; Vitamin Deficiency

Treatments: Dietary Supplement: Vitamin D (Cholecalciferol )

Study type: Interventional

Funder types: Other

Identifiers

NCT06870786

AibuIBU

Details and patient eligibility

About

ABSTRACT The aim of the study was to compare the macroscopic properties, chemical degradation levels, and fibrin network structures of T-PRF membranes obtained from systemically healthy individuals with optimal vitamin D levels, with vitamin D-deficient individuals who reached optimal levels after three months of vitamin D supplementation.

A total of 48 participants, 24 with vitamin D deficiency and 24 with optimal vitamin D levels, were included in the study. The macroscopic properties (weight, width, length, density), chemical degradation levels and fibrin network structures of the obtained T-PRF membranes were evaluated using scanning electron microscopy (SEM) and light microscopy.

Full description

Blood samples taken from individuals with the help of a 20 ml syringe were immediately transferred to 2 10 ml titanium tubes and placed opposite each other to ensure balance in the tabletop centrifuge device in order to be properly separated in the centrifuge process. To obtain T-PRF, it was centrifuged in the IntraSpin™ device at 2700 rpm centrifugal force for 12 minutes.

After centrifugation, the fibrin in the middle of the 3 layers formed in the titanium tube was held and pulled with the help of a press. Then, the red layer in the lower layer, where erythrocytes were dense, was separated from the fibrin layer with the help of scissors and the membrane was obtained by pressing for 2 minutes using the own weight of the PRF box.

Enrollment: 24 patients

Sex: All

Ages: 18 to 65 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Individuals aged 20-55
• Being systemically healthy
• Not using any medication regularly
• Not taking additional vitamin D from outside
• Patients who met the conditions of not having used antibiotics for the last three months were included in the study.

Exclusion criteria

• People who use cigarettes (tobacco) and alcohol
• Those with platelet function and clotting disorders
• Those who are pregnant or breastfeeding
• Those who use medications that affect the natural clotting process
• Those with a history of infection in the last three months
• Those receiving immunosuppressive therapy
• Those who do not consent to volunteer

Trial design

Primary purpose: Other

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: Double Blind

---

24 participants in 2 patient groups

with vitamin D deficiency

Experimental group

Description:

To obtain T-PRF membranes, blood was taken from people diagnosed with vitamin D deficiency. After 3 months, blood was taken from individuals taking vitamin D again to obtain T-PRF membranes.

Treatment:

Dietary Supplement: Vitamin D (Cholecalciferol )

Vitamin D level is optimal

No Intervention group

Description:

Blood was taken from individuals with optimum vitamin D levels to obtain T-PRF membranes