Effects of a High-fat Meal on Exercise-mediated Sympatholysis

University of Guelph

Status: Completed

Conditions: Diet, Healthy

Treatments: Dietary Supplement: Beetroot drink; Dietary Supplement: Placebo; Dietary Supplement: Control

Study type: Interventional

Funder types: Other

Identifiers

NCT06186050

23-01-017

Details and patient eligibility

About

The goal of this trial is to examine the effects of a single high-fat meal with or without the co-ingestion of dietary nitrate on exercise-mediated sympatholysis. The main questions it aims to answer are:

1. What are the mechanisms responsible for reduced exercise-mediated sympatholysis following a high-fat meal
2. Can dietary nitrate prevent the declines in exercise-mediated sympatholysis

Participants will be asked to complete three study visits in a randomized order:

1. Low-fat meal
2. High-fat meal
3. High-fat meal plus dietary nitrate

Exercise-mediated sympatholysis will be measured using Doppler ultrasound via a protocol involving rhythmic handgrip exercise and a lower body negative pressure stimulus. Sympatholysis will be assessed before each meal, and at 1, 2, and 3 hours post-prandial.

Enrollment: 15 patients

Sex: All

Ages: 18 to 30 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• 18-30 years of age
• Healthy, free of known cardiovascular, metabolic, or musculoskeletal disease

Exclusion criteria

• Diagnosed cardiovascular or metabolic disease, injury preventing handgrip exercise, prescription of chronic medications other than oral contraceptives.
• Inability to abstain from drugs (except oral contraceptives), caffeine, alcohol, strenuous physical activity, mouthwash, and multivitamins prior to study

Trial design

Primary purpose: Basic Science

Allocation: Randomized

Interventional model: Crossover Assignment

Masking: Double Blind

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15 participants in 3 patient groups, including a placebo group

High-fat meal with Beetroot Drink

Active Comparator group

Description:

2 sausage egg McMuffins and a hashbrown from McDonalds and 10g of beetroot powder mixed in 250 mL cold tap water drink

Treatment:

Dietary Supplement: Beetroot drink

High-fat meal with Placebo Drink

Placebo Comparator group

Description:

2 sausage egg McMuffins and a hashbrown from McDonalds and Mio water flavouring in 250 mL cold tap water. Trace amounts of sugar and sodium were mixed to match the beetroot supplement

Treatment:

Dietary Supplement: Placebo

Low-fat meal control

Sham Comparator group

Description:

Kelloggs cornflakes (110g), greek yogurt (400g), skim milk (500 mL), and orange juice (250 mL). Energy-matched to the high-fat meal

Treatment:

Dietary Supplement: Control