Effects of Exercise on Fructose Metabolism (FruEx2)

University of Lausanne (UNIL)

Status: Completed

Conditions: Healthy Subjects

Treatments: Other: exercise; Dietary Supplement: glucose; Dietary Supplement: fructose

Study type: Interventional

Funder types: Other

Identifiers

NCT01866215

University of Lausanne

Details and patient eligibility

About

A high fructose diet increases fasting and post-prandial triglyceride (TG) concentrations in sedentary healthy human subjects.These effects may be secondary to fructose-induced hepatic de novo lipogenesis. Recent evidence indicate that exercise can prevent fructose induced dyslipidemia.This study will evaluate

1. how exercise effects the metabolic fate of oral fructose 1a) when exercise is performed before fructose ingestion 1b) when exercise is performed after fructose ingestion Metabolic effects of exercise will be assessed in healthy male subjects by measuring fructose oxidation (13CO2 production), fructose conversion into glucose (13C glucose concentrations in blood) and hepatic fructose conversion into lipid (13C palmitate-very low density lipoprotein (VLDL) concentrations in blood) after ingestion of 13C-labelled fructose meals
2. how fructose and protein modulate muscle glycogen and intramyocellular lipid repletion after exercise Healthy male subjects will be fed various fructose, glucose, lipid and whey protein meals after a glycogen/intramyocellular lipid depleting exercise. The effects of meals' composition will be assessed after 24 hours by measuring intramyocellular lipids and glycogen using proton-magnetic resonance spectroscopy (MRS).

Enrollment: 8 patients

Sex: Male

Ages: 18 to 40 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• males
• 18-40 years old
• 19 kg/m2>BMI>25kg/m2
• moderate to high usual physical activity
• non-smokers

Exclusion criteria

• family history of diabetes mellitus
• ECG anomaly
• any medication
• participation to blood spending or other clinical study in the 3 months before the beginning of this study
• consumption of drugs
• consumption of more than 50g alcool/week
• family history of food intolerance

Trial design

Primary purpose: Basic Science

Allocation: Randomized

Interventional model: Crossover Assignment

Masking: None (Open label)

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8 participants in 5 patient groups

Study1a

Experimental group

Description:

exercice performed 90 min before 13C fructose meal ingestion

Treatment:

Other: exercise

study 1b

Experimental group

Description:

exercise performed 90 min after 13C fructose meal ingestion

Treatment:

Other: exercise

study 1c

No Intervention group

Description:

no exercise

study 2a

Experimental group

Description:

meals containing fructose, cream and whey proteins over 24 hours after a glycogen/intramyocellular lipid depleting exercise

Treatment:

Dietary Supplement: fructose

study 2b

Active Comparator group

Description:

meals containing glucose, cream and whey proteins over 24 hours after a glycogen/intramyocellular lipid depleting exercise

Treatment:

Dietary Supplement: glucose