Effects of Fermented Red Clover on Muscle Strength and Muscle Mass

University of Aarhus

Status: Completed

Conditions: Estrogen Deficiency

Treatments: Dietary Supplement: Red clover; Dietary Supplement: Placebo

Study type: Interventional

Funder types: Other

Identifiers

NCT04154306

Estrogen_2019

Details and patient eligibility

About

The project is designed as a randomized controlled, double-blinded, cross-over trial, including 10 healthy postmenopausal women consuming fermented red clover extract or placebo twice daily over a two-week period. After the first trial (two weeks), participants will undergo a two-week washout period, after which they will receive the opposite trial drink for two weeks. The trial period runs over six weeks. Before each of the two trial rounds, participants will meet in the laboratory and perform muscle strength tests (trial days 1 and 3). After each trial round (trial days 2 and 4), participants will meet fasting in the laboratory, and a blood test and a muscle biopsy will be taken initially. Next, the subject consumes the test drink (placebo or red clover extract) before the subject performs a strength training session with one leg. Right after the training session, the subject consumes a protein drink. Three hours after the protein drink, another muscle biopsy is taken. During the period from the protein drink to the final biopsy, the subject performs two muscle strength tests (respectively grip strength and maximum upper arm muscle strength (biceps)). The primary target parameter is muscle protein signaling measured in the muscle tissue samples using the western blotting method.

Enrollment: 10 patients

Sex: Female

Ages: 40 to 65 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• maximum of 5 years since last menstrual bleeding
• Minimum of 6 months since last menstrual bleeding

Exclusion criteria

• Performed systematic resistance training within the last 6 months
• Smoker
• Using any form of medicine influencing the effects of resistance training

Trial design

Primary purpose: Other

Allocation: Randomized

Interventional model: Crossover Assignment

Masking: Double Blind

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10 participants in 2 patient groups, including a placebo group

Placebo

Placebo Comparator group

Treatment:

Dietary Supplement: Placebo

Red clover

Experimental group

Treatment:

Dietary Supplement: Red clover