Effects of Free Fatty Acids and 3-hydroxybutyrate on Protein, Glucose, Lipid Metabolism and Intracellular Signals.

University of Aarhus

Status: Completed

Conditions: Systemic Inflammatory Response Syndrome

Treatments: Other: Endotoxin, US standard reference E.coli

Study type: Interventional

Funder types: Other

Identifiers

NCT01752348

1-10-72-530-12 (Other Identifier)

11422796

Details and patient eligibility

About

The investigators hypothesize that the investigators can detect protein-sparing effects of administration of the ketone 3-hydroxybutyrate and free fatty acids during simulation of an acute inflammatory disease. The investigators use the infusion of endotoxin, US standard reference E.coli in healthy subjects as a model for inflammation / infection and to evaluate the effect on protein metabolism using different tracers and the investigators can measure the various intracellular signaling pathways of selected muscle and adipose tissue.

Enrollment: 10 patients

Sex: Male

Ages: 20 to 40 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• 20 < body mass index < 30 kg/m2.
• written and orally informed consent before enrollment.

Exclusion criteria

• participation in other studies using radioactive tracers or other exposure to radiation (X-rays, scintigraphy etc).
• allergy to soy products or eggs
• diabetes, any type
• epilepsy
• ongoing infection
• immune deficiency
• cardiovascular disease
• dysregulated hypertension
• primary muscles disease, congenital or acquired

Trial design

Primary purpose: Basic Science

Allocation: Non-Randomized

Interventional model: Single Group Assignment

Masking: Single Blind

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10 participants in 4 patient groups, including a placebo group

Placebo (isotonic saline)

Placebo Comparator group

Description:

Endotoxin + isotonic saline. Reference for model of acute inflammatory illness

Treatment:

Other: Endotoxin, US standard reference E.coli

Acipimox + Placebo (isotonic saline)

Experimental group

Description:

Endotoxin + Acipimox + Placebo (isotonic saline). Intervention: blockage of endogenous lipolysis.

Treatment:

Other: Endotoxin, US standard reference E.coli

Acipimox + free fatty acids

Experimental group

Description:

Endotoxin + Acipimox + free fatty acids. Intervention: free fatty acids

Treatment:

Other: Endotoxin, US standard reference E.coli

Acipimox + 3-hydroxybutyrate

Experimental group

Description:

Endotoxin + Acipimox + 3-hydroxybutyrate. Intervention: 3-hydroxybutyrate

Treatment:

Other: Endotoxin, US standard reference E.coli