Effects of Milk Proteins on Protein Synthesis in Healthy Male Subjects Fed a Hypercaloric, High Sucrose Diet (FruMilk)

University of Lausanne (UNIL)

Status: Completed

Conditions: Obesity

Treatments: Other: 7-day overfeeding

Study type: Interventional

Funder types: Other

Identifiers

NCT02168218

218/13

Details and patient eligibility

About

This study aims at assessing the hypothesis that dietary protein content is a major factor regulating lean body mass gain and energy expenditure during overfeeding, and thus long term body weight gain

To test this hypothesis, healthy normal weight male and female subjects will before and after a 7-day hypercaloric, high-sucrose diet (+40% excess energy as sucrose) and either a low (5% total energy) or a high (20% total energy) protein intake. Each subject will be studied with both low- and high protein diets according to a randomized, crossover study. On each occasion the following measurements will be done in basal conditions (after 2-day isoenergetic, controled diet) and at the end of the 7-day overfeeding:

• whole-body protein turnover, oxidation and synthesis in fasting conditions and fed conditions (13C-labelled leucine)
• intrahepatic and intramuscular fat concentration (1H-Magnetic Resonance Spectroscopy)
• Energy metabolism in fasted and fed conditions (indirect calorimetry)
• plasma concentration of glucose, non-esterified fatty acids, total triglyceride, very-low density lipoprotein (VLDL)-triglyceride, insulin, glucagon, growth hormone (GH), insulin-like growth factor 1 (IGF1), insulin-like growth factor binding protein (IGFBP) 1,2 and 3 in fasting and fed conditions The effects of high-protein and low-protein sucrose overfeeding on whole body protein synthesis will be compared using two-way ANOVA; relationships between changes in whole body protein synthesis on one hand, and intrahepatic/intramuscular fat concentrations, total energy expenditure, and plasma concentration of metabolic variables on the other hand, will be evaluated by linear regression analysis

Enrollment: 12 patients

Sex: All

Ages: 18 to 30 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• good apparent health
• low physical activity
• non-smokers

Exclusion criteria

• vegans or following any special diet
• consumption of > 3 dl/day sweetened beverages
• changes in body > +/- 3Kg over the past 3 months
• contra-indications to magnetic resonance spectroscopy
• drug or substance abuse
• consumption > 10g/day alcohol
• lactose intolerance

Trial design

Primary purpose: Basic Science

Allocation: Randomized

Interventional model: Crossover Assignment

Masking: None (Open label)

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12 participants in 2 patient groups

high protein

Experimental group

Description:

20% protein diet

Treatment:

Other: 7-day overfeeding

low protein

Active Comparator group

Description:

7.5% protein diet

Treatment:

Other: 7-day overfeeding