Effects of Probiotic Ayran on Gingivitis

Çukurova University

Status: Completed

Conditions: Probiotics; Inflamed Gums; Gingivitis

Treatments: Dietary Supplement: Placebo ayran drink; Dietary Supplement: probiotic ayran drink

Study type: Interventional

Funder types: Other

Identifiers

NCT06437925

Cukurova University-1

Details and patient eligibility

About

Objectives: This study investigates the effects of daily consumption of probiotic ayran drink on gingival inflammation and the development of experimental gingivitis.

Methods: A total of 54volunteer students were included in the present randomized, double-blind, placebo-controlled trial.The participants were divided randomly into two groups; The Control group consisted of 27 participants who consumed placebo ayran, while the 27 participants of the Test group consumed probiotic ayran (containing Lactobacillus acidophilus and Bifidobacterium bifidum) for 42 days twice a day.After 42 days, mechanical plaque control was interrupted for 5 days. The clinical parameters of gingivitis; Plaque index (PI), gingival index (GI), probing bleeding (BOP), probing depth (PPD) were recorded at baseline, day 42 (beginning of experimental gingivitis) and day 47 (the end of experimental gingivitis). At the same time points, gingival crevicular fluid had been collected for analysis of matrix metalloproteinase - 8 (MMP-8).

Full description

The most used and studied probiotics in the periodontal literature are lactobacillus and bifidobacterium. The current study aims to observe the effects of daily ayran consumption containing a probiotic combination of Lactobacillus acidophilus and Bifidobacterium bifidum on plaque development and gingival inflammation in an experimental gingivitis model of healthy individuals. The null hypothesis of this study using of probiotics has no additional benefit for periodontal health.

This study evaluates the effects of daily consumption of ayran containing a probiotic combination of Lactobacillus Acidophilus and Bifidobacterium Bifidum on plaque development and gingival status in healthy individuals with experimental gingivitis.

Enrollment: 54 patients

Sex: All

Ages: 18 to 25 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Systemic healthy
• Subjects with gingivitis defined as a BOP sites ≥ 10% and PD ≤ 3 mm
• No radiographic bone loss
• Non-smoking participants

Exclusion criteria

• History of using antibiotics or anti-inflammatory drugs or probiotic preparations or food supplements in the last 6 months,
• Undergoing orthodontic treatment,
• Active carious lesions
• Mouth breathing
• History of allergy for milk or fermented milk products.
• Taking medications affecting the gingiva and/or oral mucosa

Trial design

Primary purpose: Prevention

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: Double Blind

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54 participants in 2 patient groups, including a placebo group

Probiotic ayran drink

Experimental group

Description:

The participants in this experimental group will use probiotic containing ayran for 42 days and then will be subjected to 5 days of experimental gingivitis.

Treatment:

Dietary Supplement: probiotic ayran drink

Placebo ayran drink

Placebo Comparator group

Description:

The participants in this control group will use control ayran without probiotics for 42 days and then will be subjected to 5 days of experimental gingivitis.

Treatment:

Dietary Supplement: Placebo ayran drink