Effects of Propolis Flavonoids on TLR4 Signaling and Inflammation in Human PDLSCs In Vitro

Cairo University (CU)

Status: Not yet enrolling

Conditions: Inflammatory Cytokine Expression

Treatments: Other: no medication added; Drug: Flavonoid extract from propolis

Study type: Observational

Funder types: Other

Identifiers

NCT07367451

12/2025

Details and patient eligibility

About

This in-vitro study evaluated the effects of a flavonoid-rich propolis extract on Toll-like receptor 4 (TLR4) signaling and inflammatory cytokine expression in human periodontal ligament stem cells (PDLSCs). PDLSCs were treated with propolis flavonoids under baseline conditions and following lipopolysaccharide (LPS)-induced inflammation, with untreated cells serving as controls. TLR4 gene expression was analyzed using PCR, while levels of pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α) were quantified by ELISA. Cell viability and cytotoxicity at different extract concentrations were assessed using the MTT assay. The study aimed to determine the immunomodulatory and cytocompatible potential of propolis flavonoids in regulating inflammatory responses in PDLSCs.

Enrollment: 20 estimated patients

Sex: All

Volunteers: No Healthy Volunteers

Inclusion criteria

• invitro study on Human Periodontal Ligament Stem Cells

Exclusion criteria

• none

Trial design

20 participants in 2 patient groups

Isolated PDLSCs, and adding Flavonoid extract from propolis

Treatment:

Drug: Flavonoid extract from propolis

Isolated PDLSCs, no material added

Treatment:

Other: no medication added