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Effects of Protein Hydrolysate Supplementation Influencing Strength Recovery and Expression of Muscle Damage Markers Post-exercise

N

Nuritas

Status

Completed

Conditions

Muscle Injury

Treatments

Other: SMCC
Dietary Supplement: Elio

Study type

Interventional

Funder types

Other
Industry

Identifiers

NCT05159375
PN20.004.01

Details and patient eligibility

About

A randomised placebo controlled, double-blind, 30 Volunteer trial of Elio™ administered 2.4 grams per day in assessing its effects on post-exercise strength recovery and increases in markers associated with muscle injury and exertion.

Full description

Periods of intense physical activity and exertion can lead to disruption of normal muscle homeostasis and the resultant muscle injury and recovery can impact upon quality of life especially in adult populations. Recovery post-exercise is therefore important to all active people.

There is a link between high-intensity exercise and reduced power and performance in the following exercise sessions. The effect on performance is measures as a reduced muscle force production or loss in strength and can correlate with delayed onset muscle soreness (DOMS). The reduced power/performance seen 24 hours - 7days post-exercise is affected by recovery methods applied, individual fitness levels and intensity of exercise injury.

Several theories have been proposed to explain the mechanisms underlying DOMS. These include inflammation and muscle damage.

Exercise-induced muscle damage is a transient phenomenon caused by unfamiliar, damaging exercise and is characterized by structural damage to myofibers and secondary inflammation. Signs and symptoms often persist for several days after exercise and typically include muscle soreness, elevated blood levels of intramuscular enzymes such as creatine kinase (CK), lactate dehydrogenase (LDH) and myoglobin (MB) that often result in elevations in circulating markers of inflammation such as C-reactive protein (CRP) and various interleukins. To date, extensive research has been published that explore the many recovery strategies purported to minimize indirect markers of muscle damage.

However, from a nutritional standpoint the results are weak. It is therefore important to develop nutritional products that can lessen the impact of these injuries' individuals succumb to or expedite their recovery.

Nutritional recovery strategies purported to minimise indirect markers of muscle damage and improve the inflammatory response can positively influence the recovery process after damaging exercise.

These strategies could in turn be used to prime the muscle for physical challenges.

Furthermore, there is a clear absence of natural approaches with proven evidence to address DOMS and the resulting performance declines.

Therapies such as non-steroidal anti-inflammatory drugs (NSAIDs) can be taken in the short term to reduce DOMS associated pain and inflammation. However, these drugs do not address performance strength loss linked to DOMS.

Acute injuries to discrete muscle groups can be indirectly detected by an array of systemic biomarkers, which become elevated at various time-points following the event.

This array of biomarkers represents key biochemical processes in muscle homeostasis, cellular integrity, mitochondrial function and inflammation.

Preliminary investigations in vitro and in vivo suggest that the administration of peptides isolated from the protein extract of fava beans, which constitute Elio™, can augment muscle protein synthesis (i.e. phospo-S6) and attenuate proteolytic signaling (i.e. Fbxo32 and Trim63) and surrogate inflammatory markers (TNFα and IL6). Thus, supporting the use of this hydrolysate in maintaining muscle homeostasis and function.

With the present study the investigators will investigate whether daily dietary supplementation of Elio™, over the course of 15 days can prime the muscle by improving strength recovery and attenuating the expression of systemic markers associated with muscle injury and over exertion following resistance type exercise in a healthy male population.

Objectives

To determine the effect of Elio™ supplementation, a protein hydrolysate derived from fava bean protein extract, on strength recovery and markers of muscle health, injury and function following strenuous resistance type exercise in male volunteers, aged between 30 and 45 years old following 17 days of supplementation.

Enrollment

44 patients

Sex

Male

Ages

30 to 45 years old

Volunteers

Accepts Healthy Volunteers

Inclusion criteria

  1. Males between 30 and 45 years of age
  2. Participants agree to comply with study procedures. Participants agree to comply with study procedures.
  3. Participants agree to abstain from taking additional supplements throughout the testing period, with particular emphasis placed upon protein-based products
  4. Participants agree to maintain their normal diet and exercise routine throughout the study
  5. BMI between 18.5 to 29.9 kg/m2
  6. Participants agree to refrain from consuming alcohol in the 48 hrs leading up to a test day.
  7. Willingness to complete questionnaires, records and diaries associated with the study and to complete all clinic visits.
  8. Provide voluntary, written, informed consent to participate in the study.
  9. Refrain from any sort of exhaustive physical exercise from 48 hrs prior to each test or blood draw
  10. Healthy as determined by medical examination at screening visit
  11. Willingness to complete food diaries during the study
  12. Must have a smart phone to use the Nutritics App
  13. Non-smoker

Exclusion criteria

  1. Alcohol or drug abuse in past year

  2. Participation in any other clinical trial in the last 3 months from time of randomisation

  3. Volunteer has a known allergy to the test material's active or inactive ingredients

  4. Volunteers with unstable medical conditions

  5. Any complaints that could interfere with ability to exercise

  6. Individuals who are cognitively impaired and/or who are unable to give informed consent

  7. Any co-morbidities interacting with mobility or muscle metabolism of the lower limbs (e.g., arthritis, spasticity/rigidity, all neurological disorders and paralysis)

  8. Creatine supplements, anticoagulants, corticosteroids, growth hormones,

    testosterone, immunosuppressants, or exogenous insulin over the previous three months

  9. Presence or history of neurological disorders or significant psychiatric illness.

  10. Any other condition which in the Investigator's opinion may adversely affect the volunteer's ability to complete the study or its measures or which may pose significant risk to the volunteer

  11. Participation in resistance or aerobic exercise within 48 hours of the test days

  12. Participation in > 3 High-intensity Exercise sessions per Week

  13. Undertake no recovery methods such as sea swims, foam rolling, cryotherapy or undue stretching during Days 14-17.

  14. Have been in contact with a suspected or confirmed case of Covid-19 in the previous 14 days

  15. Are Hepatitis A or B positive, HIV positive or have had a sexual partner who is infected with hepatitis or HIV

Trial design

Primary purpose

Prevention

Allocation

Randomized

Interventional model

Parallel Assignment

Masking

Single Blind

44 participants in 2 patient groups, including a placebo group

Elio (supplement under investigation)
Experimental group
Description:
2.4g of Elio administered orally daily with the first meal of the day for a 17 day period
Treatment:
Dietary Supplement: Elio
Placebo
Placebo Comparator group
Description:
2.4g of SMCC administered orally daily with the first meal of the day for a 17 day period
Treatment:
Other: SMCC

Trial contacts and locations

1

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Central trial contact

Thomas Ball; Thayse Passos

Data sourced from clinicaltrials.gov

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