Effects of Subanesthetic Isoflurane/Sevoflurane in 60% Oxygen on Clinical In-vitro Experimental Sepsis

Air Force Military Medical University of People's Liberation Army

Status: Unknown

Conditions: Sepsis

Treatments: Drug: Isoflurane; Drug: oxygen; Drug: Sevoflurane

Study type: Interventional

Funder types: Other

Identifiers

NCT02185118

Chinese NSFC (Other Grant/Funding Number)

NSFC-81171839

Details and patient eligibility

About

Sepsis is a major cause of death in intensive care units. Despite the investigators improved understanding, which has reduced the risk of dying with sepsis, the number of people who die each year continues to increase due to an overall increase in the number of cases.In our previous study, the investigators have showed that 100% oxygen or 0.5 minimum alveolar concentration (MAC) isoflurane/sevoflurane in 60% oxygen protect mouse macrophage cell line against in-vitro sepsis induced by lipopolysaccharide (LPS).

In this study, the investigator hypothesized that treatment of 100% oxygen or 0.5 MAC isoflurane/sevoflurane in 60% oxygen protected against clinical in-vitro models of sepsis induced by LPS or plasma from septic patients.

Full description

100% oxygen or 0.5 MAC isoflurane/sevoflurane in 60% oxygen would inhibit increases of tumor necrosis factor (TNF)-alpha, interleukin-1beta, interleukin-6 in the cell culture supernatant after stimulation of LPS or plasma from septic patients, and also inhibit the nuclear location of nuclear factor-kappa B p65 subunit.

Enrollment: 50 estimated patients

Sex: All

Ages: 18+ years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Human PBMCs were only isolated from the adult patients without sepsis/SIRS/infectious diseases.
• The plasma for induction of clinical in-vitro sepsis, was only isolated from adult patients with sepsis.

Exclusion criteria

• Patients who had been selected for other clinical trials in the 3 months before.

Trial design

Primary purpose: Treatment

Allocation: N/A

Interventional model: Single Group Assignment

Masking: None (Open label)

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50 participants in 1 patient group

oxygen plus isoflurane/sevoflurane

Experimental group

Description:

All human peripheral blood mononuclear cells (PBMCs) were from patients with non sepsis/non SIRS/non infection. The above cells were treated with oxygen or oxygen plus isoflurane/sevoflurane after stimulation of lipopolysaccharide/plasma from septic patients.

Treatment:

Drug: oxygen

Drug: Sevoflurane

Drug: Isoflurane