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Efficacy Study of Different Laboratory Management Strategies and Drug Regimens in HIV-infected Children in Africa (ARROW)

U

United Kingdom Research and Innovation (UKRI)

Status and phase

Completed
Phase 4

Conditions

Human Immunodeficiency Virus

Treatments

Other: Stopped cotrimoxazole prophylaxis
Drug: Twice-daily ABC+3TC
Other: Clinically Driven Monitoring (CDM)
Drug: Arm C: ZDV+ABC+3TC+NNRTI->ZDV+ABC+3TC maintenance
Drug: Arm B: ZDV+ABC+3TC+NNRTI->ABC+3TC+NNRTI maintenance
Drug: Once-daily ABC+3TC
Drug: Continued cotrimoxazole prophylaxis
Other: Laboratory plus Clinical Monitoring (LCM)
Drug: Arm A: ABC+3TC+NNRTI

Study type

Interventional

Funder types

Other
Industry

Identifiers

NCT02028676
G0300400 (Other Grant/Funding Number)
24791884 (Registry Identifier)

Details and patient eligibility

About

The two original objectives were to determine in HIV-infected children initiating antiretroviral therapy (ART):

  1. Whether clinically driven monitoring (CDM) will have a similar outcome in terms of disease progression or death as routine laboratory and clinical monitoring (LCM) for toxicity (haematology/biochemistry) and efficacy (CD4)?

  2. Whether induction with four drugs from two ART classes followed by maintenance with three drugs after 36 weeks be more effective than a continuous non-nucleoside reverse transcriptase inhibitors (NNRTI)-based triple drug regimen in terms of CD4 and clinical outcome?

    Two secondary objectives were to determine

  3. Whether changing from twice daily lamivudine+abacavir to once daily lamivudine+abacavir after 48 weeks on ART will have a similar outcome in terms of virological suppression and will result in improvements in adherence to ART?

  4. Whether stopping daily cotrimoxazole prophylaxis in children over 3 years of age who have been on ART for at least 96 weeks has a similar outcome in terms of hospitalisation or death as continuing daily cotrimoxazole?

Full description

The ARROW (AntiRetroviral Research fOr Watoto) protocol describes an open-label randomised trial primarily evaluating two strategic approaches for management of antiretroviral therapy (ART) in 1200 symptomatic HIV-infected infants and children initiating ART following WHO guidelines in Uganda and Zimbabwe. The first strategy compares clinically driven monitoring (CDM) with laboratory plus clinical monitoring (LCM). In both groups, tests for toxicity (standard haematology and biochemistry panels) and efficacy (lymphocyte subsets including CD4 count) will be done every 12 weeks. In LCM, all results will be returned for patient management. In CDM, physicians may request results from routine haematology/biochemistry panels if needed for clinical management, but results will not be returned routinely, and lymphocyte subsets will never be returned. Extra laboratory tests may be requested outside of the scheduled visits at any time in either group (except for lymphocyte subsets in CDM). The second strategy compares a continuous WHO-recommended first-line ART three-drug two-class regimen, comprising two Nucleoside Reverse Transcriptase Inhibitors (NRTIs) plus one Non-Nucleoside Reverse Transcriptase Inhibitor (NNRTI), with induction with four drugs (two classes) for 36 weeks followed by maintenance with three drugs. After at least 36 and 96 weeks on ART respectively, two further randomisations will assess simplification strategies which could improve long-term ART adherence (i) once versus twice daily lamivudine+abacavir NRTI drugs (ii) stopping versus continuing daily cotrimoxazole prophylaxis.

Enrollment

1,206 patients

Sex

All

Ages

3 months to 17 years old

Volunteers

No Healthy Volunteers

Inclusion and exclusion criteria

For initial randomisation to CDM vs LCM, and to ART induction strategy:

Inclusion Criteria:

  1. Children should have an adult carer in the household who is either:

    • participating in the DART trial OR
    • being treated with ART OR
    • HIV positive but not yet needing treatment but with access to a treatment programme when ART is required OR
    • HIV negative. Children of DART participants should have first priority on any available remaining slots to enter ARROW.
  2. Parents or guardians, and children where appropriate according to age and knowledge of HIV status, must be willing and able to give informed consent for randomisation to CDM or LCM and to first-line ART strategy.

  3. Participants must have a confirmed documented diagnosis of HIV-1 infection:

    1. For children aged under 18 months: two separate peripheral blood specimens from different days, both results being positive with HIV-DNA polymerase chain reaction (PCR).
    2. For children aged 18 months or over: antibody positive serology by ELISA test (confirmed by licensed second ELISA or Western Blot) or WHO approved rapid test (performed in series) both on the same sample. Any child previously tested at another clinic should have a repeat test at an ARROW screening laboratory to confirm their status.
  4. Age 3 months to 17 years (13-17 years to be capped at 10%)

  5. ART naïve (except for exposure to perinatal ART for the prevention of mother-to-child HIV transmission).

  6. Meeting criteria for requiring ART according to WHO stage and CD4 percent or count:

    • WHO paediatric clinical stage IV disease: treat regardless of CD4 percent or count

    • WHO paediatric clinical stage III disease:

      • <12 months: treat all
      • >12 months: treat all children irrespective of the CD4 percent or count; however, in children aged > 12 months with tuberculosis, lymphocytic interstitial pneumonia (LIP), oral hairy leukoplakia (OHP) or thrombocytopenia (low platelet count treat) be guided by CD4 cell assays (see below).
    • WHO paediatric clinical stage II or I disease: treat guided by CD4 percent or count

      • CD4%<25% for infants <12 months;
      • CD4%<20% for children 1-<3 years;
      • CD4% <15% for children 3-<5years;
      • CD4% <15% for children > 5years (consideration should also be taken of the CD4 count. A CD4 count <200 cells/mm3 can be used to guide starting ART and CD4 should generally be <350 cells/mm3.)

Exclusion Criteria:

  1. Cannot, or unlikely to attend regularly (e.g. usual residence too far from study centre)

  2. Likelihood of poor adherence

  3. Presence of acute infection (e.g. malaria, helminthiasis, acute hepatitis, acute pneumonia, septicaemia, meningitis). Children may be admitted after recovery of an acute infection. Children with chronic lung disease, including recurrent respiratory infections, are eligible. Children with tuberculosis (TB) will not be enrolled while on the intensive phase of anti-tuberculosis therapy, but should be re-evaluated after the intensive phase and a decision made then about starting ART (see 4 below)

  4. In receipt of medication contraindicated by ART

    • children under three years of age receiving anti-tuberculosis therapy should not be enrolled (as they will have to receive nevirapine).
    • on chemotherapy for malignancy
  5. Laboratory abnormalities which are a contra-indication for the child to start ART (haemoglobin <8.5g/dL; neutrophils <0.50x109/L; aspartate transaminase (AST) or alanine transaminase (ALT) >5 x the upper limit of normal (ULN); grade 3 renal dysfunction - creatinine >1.9 x ULN).

    N.B. causes of anaemia, such as concurrent bacterial infection, malaria, helminthiasis and/or malnutrition should be investigated, and treatment for anaemia and its causes commenced prior to re-screening for eligibility.

  6. Being pregnant or breast-feeding an infant

  7. Perinatal exposure to nevirapine (either through prevention of mother-to-child transmission (pMTCT) or breastfeeding) for children aged 3 - 6 months only

Eligibility criteria for the secondary randomisation to once vs twice daily lamivudine+abacavir Inclusion criteria

  1. Participating in ARROW

  2. On ART for at least 36 weeks

  3. Currently taking lamivudine+abacavir twice daily as part of their ART regimen and expected to stay on these two drugs for at least the next 12 weeks

  4. Parents or guardians, and children where appropriate according to age and knowledge of HIV status, must be willing and able to give informed consent for randomisation to once or twice daily lamivudine+abacavir

    Exclusion criteria

  5. Likely to switch to second-line therapy in the next 12 weeks

Eligibility criteria for the secondary randomisation to stop or continue cotrimoxazole prophylaxis randomisation Inclusion criteria

  1. Participating in ARROW

  2. Aged at least 3 years

  3. Initiated ART at least 96 weeks previously, and received at least 96 weeks of ART allowing for any interruptions in ART

  4. Currently prescribed daily cotrimoxazole as primary prophylaxis

  5. Parents or guardians, and children where appropriate according to age and knowledge of HIV status, must be willing and able to give informed consent for randomisation to stop or continue daily cotrimoxazole prophylaxis

  6. If living in a malaria endemic area, has an insecticide treated bednet and prepared to use this for the child.

    Exclusion criteria

  7. Previous diagnosis of Pneumocystis jiroveci pneumonia (cotrimoxazole is secondary prophylaxis and should not be discontinued)

Trial design

Primary purpose

Treatment

Allocation

Randomized

Interventional model

Factorial Assignment

Masking

None (Open label)

1,206 participants in 9 patient groups

Clinically Driven Monitoring (CDM)
Experimental group
Treatment:
Other: Clinically Driven Monitoring (CDM)
Laboratory plus Clinical Monitoring (LCM)
Active Comparator group
Treatment:
Other: Laboratory plus Clinical Monitoring (LCM)
Arm A: abacavir (ABC)+lamivudine (3TC)+NNRTI
Active Comparator group
Description:
ABC \[abacavir\]: syrup or tablet, dosed twice-daily according to weight-bands following WHO 3TC \[lamivudine\]: syrup or tablet, dosed twice-daily according to weight-bands following WHO non-nucleoside reverse transcriptase inhibitor (NNRTI): either nevirapine or efavirenz based on availability (provided by national ART programmes in Uganda/Zimbabwe). Nevirapine syrup or tablet dosed twice-daily according to weight-bands following WHO. Efavirenz tablets dosed once-daily according to weight-bands following WHO.
Treatment:
Drug: Arm A: ABC+3TC+NNRTI
Arm B: ZDV+ABC+3TC+NNRTI->ABC+3TC+NNRTI maintenance
Experimental group
Description:
ZDV \[abacavir\]: syrup or tablet, dosed twice-daily according to weight-bands following WHO ABC \[abacavir\]: syrup or tablet, dosed twice-daily according to weight-bands following WHO 3TC \[lamivudine\]: syrup or tablet, dosed twice-daily according to weight-bands following WHO NNRTI: either nevirapine or efavirenz based on availability (provided by national ART programmes in Uganda/Zimbabwe). Nevirapine syrup or tablet dosed twice-daily according to weight-bands following WHO. Efavirenz tablets dosed once-daily according to weight-bands following WHO.
Treatment:
Drug: Arm B: ZDV+ABC+3TC+NNRTI->ABC+3TC+NNRTI maintenance
Arm C: ZDV+ABC+3TC+NNRTI->ZDV+ABC+3TC maintenance
Experimental group
Description:
ZDV \[abacavir\]: syrup or tablet, dosed twice-daily according to weight-bands following WHO ABC \[abacavir\]: syrup or tablet, dosed twice-daily according to weight-bands following WHO 3TC \[lamivudine\]: syrup or tablet, dosed twice-daily according to weight-bands following WHO NNRTI: either nevirapine or efavirenz based on availability (provided by national ART programmes in Uganda/Zimbabwe). Nevirapine syrup or tablet dosed twice-daily according to weight-bands following WHO. Efavirenz tablets dosed once-daily according to weight-bands following WHO.
Treatment:
Drug: Arm C: ZDV+ABC+3TC+NNRTI->ZDV+ABC+3TC maintenance
Once-daily ABC+3TC
Experimental group
Description:
ABC \[abacavir\]: syrup or tablet, dosed once-daily according to weight-bands following WHO 3TC \[lamivudine\]: syrup or tablet, dosed once-daily according to weight-bands following WHO
Treatment:
Drug: Once-daily ABC+3TC
Twice-daily ABC+3TC
Active Comparator group
Description:
ABC \[abacavir\]: syrup or tablet, dosed twice-daily according to weight-bands following WHO 3TC \[lamivudine\]: syrup or tablet, dosed twice-daily according to weight-bands following WHO
Treatment:
Drug: Twice-daily ABC+3TC
Continued cotrimoxazole prophylaxis
Active Comparator group
Description:
Once-daily doses 5-\<15 kg: 200 mg of trimethoprim + 40 mg sulfamethoxazole 15-\<30 kg: 400 mg trimethoprim + 80 mg sulfamethoxazole \>=30 kg: 800 mg trimethoprim + 160 mg sulfamethoxazole
Treatment:
Drug: Continued cotrimoxazole prophylaxis
Stopped cotrimoxazole prophylaxis
Experimental group
Description:
Children had been taking once-daily cotrimoxazole prophylaxis since at least ART initiation. Children randomised to this experimental arm stopped taking cotrimoxazole prophylaxis.
Treatment:
Other: Stopped cotrimoxazole prophylaxis

Trial contacts and locations

4

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Data sourced from clinicaltrials.gov

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