Embryo Development in Microfluidics System of Culture

Federal University of São Paulo

Status and phase: Unknown; Phase 2

Conditions: Infertility

Treatments: Device: SMART dynamic system of embryo culture; Device: Static culture

Study type: Interventional

Funder types: Other

Identifiers

NCT01003548

0405/08

Details and patient eligibility

About

Embryo culture is a cornerstone of in vitro fertilization treatments. Usually, embryos are cultured in microdrops of culture media in incubators with controlled temperature, humidity and atmosphere. However, these culture systems are static, while in vivo the initial growth of the embryo takes place in the uterine tube and they are submitted to a dynamic environment. The aim of this study is to compare the embryo development on days 2 and 3 of culture morphological features of embryos under static and dynamic(microfluidics) systems of culture.

Full description

Embryo culture is a cornerstone of in vitro fertilization treatments. Usually, embryos are cultured in microdrops of culture media in incubators with controlled temperature, humidity and atmosphere. However, these culture systems are static, while in vivo the initial growth of the embryo takes place in the uterine tube and they are submitted to a dynamic environment. The aim of this study is to compare the embryo development on days 2 and 3 of culture morphological features of embryos under static and dynamic(microfluidics) systems of culture.

Enrollment: 400 estimated patients

Sex: Female

Ages: 21 to 35 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Age between 21 and 35 years old
• No more than 1 previous IVF failure
• Absence of physical conditions that can interfere in oocyte quality
• At least 8 zigotes 24 hours after fertilization

Exclusion criteria

• Use of epididimary or testicular sperm
• Sperm or egg donation
• Untreated systemic diseases

Trial design

400 participants in 2 patient groups

Static culture

Active Comparator group

Description:

Embryos individually cultured in microdrops of 40 microliters of G-IVFplus series V

Treatment:

Device: Static culture

Smart plataform

Experimental group

Description:

Embryos culture in dynamics microfluidic culture system

Treatment:

Device: SMART dynamic system of embryo culture