EMT in Peritoneal Dialysis Patients

University of Colorado Denver (CU Denver)

Status: Completed

Conditions: Peritoneal Dialysis Complication

Treatments: Diagnostic Test: Isolation of peritoneal mesothelial cells

Study type: Observational

Funder types: Other

Identifiers

NCT03458819

18-0401

Details and patient eligibility

About

Approximately 10-11% of end stage kidney disease patients worldwide utilize peritoneal dialysis (PD) as their method of renal replacement therapy. Over time, the peritoneal membrane often undergoes anatomic and functional changes due to the process of epithelial to mesenchymal transition (EMT). EMT is characterized by increases in pro-inflammatory and pro-angiogenic cytokines. In this process, the mesothelial cells lining the peritoneal membrane are denuded and change their morphology to one more closely resembling fibroblasts. These fibroblasts invade the submesothelial zone of the peritoneal membrane resulting in marked fibrosis, and the pro-angiogenic cytokines cause an increase in neovascularization. Jointly, these processes culminate in impaired function of the peritoneal membrane and often limit the duration of effective PD therapy.

In vitro studies in cultured human peritoneal mesothelial cells (HPMCs) and in vivo studies in rodent models of PD have demonstrated that the use of active Vitamin D receptor agonists or statins may attenuate this process of EMT. These are both classes of drugs that are commonly in use by patients on PD. The investigators goal is to determine whether either or both of these drugs might attenuate the process of EMT in patients performing PD.

Full description

Patients will perform a standard 2 Liter overnight dwell with the usual 2.5% dextrose PD solution. This will be done the night before a routinely scheduled visit to the home dialysis clinic. In the clinic the fluid will be drained in usual fashion and the fluid- which would otherwise be discarded- will be analyzed as below:

HPMCs will be isolated as previously described by Kinashi et al. and stored at -80 degrees until analysis. Changes in the protein expression of EMT markers such as E-cadherin, alpha smooth muscle actin (a-SMA), Snail, and fibronectin in the HPMCs will be evaluated by Western blot analysis.

Enrollment: 22 patients

Sex: All

Ages: 18 to 90 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Patients > 18 years of age
• Patients on Peritoneal dialysis, and
• Patients that meet at least one of the criteria noted in the Population Description.

Exclusion criteria

• Patients with Recent peritonitis

Trial design

22 participants in 4 patient groups

Control

Description:

Patients on neither active Vitamin D or a statin

Treatment:

Diagnostic Test: Isolation of peritoneal mesothelial cells

Vit D

Description:

Patients on active Vitamin D but not a statin

Treatment:

Diagnostic Test: Isolation of peritoneal mesothelial cells

Statin

Description:

Patients on a statin but not active Vitamin D

Treatment:

Diagnostic Test: Isolation of peritoneal mesothelial cells

D + Statin

Description:

Patients on both active Vitamin D and a statin

Treatment:

Diagnostic Test: Isolation of peritoneal mesothelial cells