Endogenous Glucose Production in Patients With Glycogen Storage Disease Type Ia (ENGLUPRO GSDIa)

University Medical Center Groningen (UMCG)

Status and phase: Completed; Phase 2; Phase 1

Conditions: Glycogen Storage Disease Type IA

Treatments: Biological: [6,6-2H2]glucose

Study type: Interventional

Funder types: Other; Industry

Identifiers

NCT04311307

NL73191.042.20

Details and patient eligibility

About

Glycogen storage disease type Ia (GSDIa) subjects retain a limited capacity for endogenous glucose production (EGP). To date, the origin of residual EGP in GSDIa patients is unknown. Either increased glycogen debranching or lysosomal glycogen breakdown can account for residual EGP in GSDIa.

Innovative treatments for GSDIa (e.g. AAV8-mediated gene therapy and mRNA therapy) are being developed.Therefore, longitudinal minimally-invasive monitoring of outcomes after therapeutic interventions in GSD Ia subjects becomes warranted.

The primary objective is to test the feasibility of EGP quantification in adult GSDIa subjects by stable isotopes after a single oral [6,6-2H2]glucose dose. Secondary objectives are to compare EGP assessed by a single oral [6,6-2H2]glucose dose (a) in GSDIa patients versus matched healthy participants, (b) among GSDIa patients, (c) in the pre-prandial state versus the fed state, (d) in the controlled hospital setting versus the home setting. Data collected from the continuous glucose monitoring data will also be compared

Full description

Study design: An investigator-initiated human pilot-study. Study population: Ten adult subjects with GSDIa and ten age and gender-matched healthy subjects.

Interventions: Three experiments will be performed for each subject. During the first in hospital experiment, two oral D-[6,6-2H2]glucose loads will be performed 2 hours before breakfast and at lunchtime, respectively. The third oral D-[6,6-2H2]glucose load will be performed at home (random time). Capillary blood samples will be collected on filter paper at specific time points after each oral D-[6,6-2H2]glucose load. During the experiments, subjects will be monitored by subcutaneous continuous glucose monitoring (CGM).

Enrollment: 20 patients

Sex: All

Ages: 16+ years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• The diagnosis should be confirmed by G6PC mutation analysis
• Age above 16 years
• Stable medical condition before the start of the test procedures

Exclusion criteria

• Age < 16 years
• Intercurrent illness
• Recent history of hospitalization due to hypoglycemia
• Pregnancy
• History suggestive of diabetes or fasting intolerance
• First grade family member with a confirmed diagnosis associated with fasting intolerance

Trial design

Primary purpose: Basic Science

Allocation: Non-Randomized

Interventional model: Parallel Assignment

Masking: None (Open label)

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20 participants in 2 patient groups

Patients

Experimental group

Description:

GSDIa patients

Treatment:

Biological: [6,6-2H2]glucose

Controls

Active Comparator group

Description:

Healthy volunteers

Treatment:

Biological: [6,6-2H2]glucose