Enteropathogenic Escherichia Coli (EPEC): Does it Have a Role in Colorectal Tumourigenesis?

Mansoura University

Status: Completed

Conditions: Colorectal Carcinoma; Enteropathogenic Escherichia Coli .

Treatments: Other: colonoscopic biopsies

Study type: Observational

Funder types: Other

Identifiers

NCT02373020

e coli

Details and patient eligibility

About

Despite the characterization of many aetiologic genetic changes. The specific causative factors in the development of sporadic colorectal cancer remain unclear. This study was performed to detect the possible role of Enteropathogenic Escherichia coli (EPEC) in developing colorectal carcinoma.

Full description

Fresh biopsy specimens have been obtained from the colonic mucosa overlying the colorectal cancer as well as from the colon of the healthy controls. Culture, genotyping and virulence of EPEC were done using (nutrient broth culture, and PCR). Strains biochemically identified as E. coli were selected from the surface of a MacConkey's plate and were serogrouped by slide agglutination tests

Enrollment: 500 patients

Sex: All

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• patients with colorectal carcinoma after have been diagnosed

Exclusion criteria

• Patients with inflammatory bowel disease (IBD) or macroscopic signs of inflammation were excluded.

Trial design

500 participants in 2 patient groups

group 1

Description:

colonoscopic biopsies from patients with colorectal cancer patients

Treatment:

Other: colonoscopic biopsies

(Group 2

Description:

colonoscopic biopsies from healthy controls

Treatment:

Other: colonoscopic biopsies