Enumeration and Function Analysis of Treg Cells in Peripheral Blood of HCC Patients Before and After Ablation Therapy

Sun Yat-sen University

Status: Unknown

Conditions: Hepatocellular Carcinoma

Treatments: Procedure: radiofrequency ablation

Study type: Observational

Funder types: Other

Identifiers

NCT01668381

2010B031600209

Details and patient eligibility

About

The purpose of this study is to determine the enumeration and function changes of regulatory t cells in peripheral blood of hepatocellular carcinoma patients before and 1 week, 4 weeks after ablation therapy.

Full description

Regulatory T cells，which are also called Treg cells，play an important role in suppressing anti-tumor immunity.Accumulated evidences indicate that Treg cells are elevated in peripheral blood,however,there are also reports that decreased Treg cells are found in hepatocellular patients.This study focuses on the changes of Treg cells ratio in peripheral blood of hepatocellular carcinoma patients before and 1 week, 4 weeks after ablation ,also its functional cytokines,such as TGF-β,IL-10,IFN-γ,and inhibition function when co-cultured with CD4+CD25-、CD8+ cells. The investigators speculate that a decreased Treg ratio will be found in patients who receive ablation therapy,and its function cytokines,moreover,CD4+CD25-、CD8+ cells' proliferation and function could be inhibited when co-cultured with Treg cells.

Enrollment: 20 estimated patients

Sex: All

Ages: 18 to 80 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• hepatocellular patients diagnosed through biopsy;or either dynamic imagine with a diagnosis of hepatocellular carcinoma and alphafetoprotein>400μg/L；or two or more dynamic imagine with a diagnosis of hepatocellular carcinoma
• Child-Pugh A or B
• well preserved renal and hematopoietic Function
• receive ablation therapy through percutaneous radiofrequency ablation or microwave ablation or ethanol injection ablation or any kind of combination of them.
• achieve complete ablation accessed by contrast-enhanced CT

Exclusion criteria

• incomplete ablation
• remote metastasis
• Child-Pugh C
• general infection
• autoimmune diseases
• suffer from other tumors concurrently or in last five years
• patients with immune deficit or infected by HIV
• receiving glucocorticoid or other medicine inhibiting immune system

Trial design

20 participants in 1 patient group

HCC patients

Description:

Hepatocellular carcinoma patients treated by radiofrequency ablation

Treatment:

Procedure: radiofrequency ablation