Epigenetic Integrity of Spermatozoa in Patients With Germinal Testicular Tumours (GAMETH)

University Hospital Center (CHU) Dijon Bourgogne

Status: Unknown

Conditions: Seminomas

Treatments: Other: Analysis of methylation profiles of numerous GSI and TE; Other: Method of protein detection by immunostaining and flow cytometry; Other: Extraction genomic DNA; Other: Analysis of methylation for the whole genome (sub-population of 15 samples from group 1)

Study type: Observational

Funder types: Other

Identifiers

NCT03262207

DESCH AOI 2016

Details and patient eligibility

About

Recent data suggest that sperm cells carry an epigenetic message during spermatogenesis and that this message is crucial for the future development of the embryo. This epigenetic signature is notably represented by methylation of genes subjected to imprinting (GSI) and the methylation of transposable elements (TE). Data on the maintenance of the imprint and of the control of TE accompanying human gametogenesis in a context of adult germinal testicular cancers, seminomas, are extremely fragmentary for tumour tissues and inexistent for gametes.

The aim of this study is to determine whether patients with seminomas in comparison with fertile men carry a higher risk of presenting epigenetic alterations affecting their gametes.

This study is based on the use of an existing collection of biological samples. 90 samples will be selected and split into 3 groups:

• Group 1: 30 sperm samples from patients with seminomatous testicular tumours
• Group 2: 30 sperm samples from fertile patients
• Group 3: 30 sperm samples from infertile patients

After treatment of the samples (thawing, cell sorting and removal of cryoprotectants), they will be analysed.

Enrollment: 90 patients

Sex: Male

Ages: 18+ years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• biological samples from persons who have provided written consent
• biological samples of patients over 18 years old
• with testicular tumours/Fertile/Infertile
• Matched for age with patients suffering from testicular tumours

Exclusion criteria

• biological samples of persons without national health insurance cover
• biological samples from patients with chronic hepatitis B or C or HIV infection

Trial design

90 participants in 3 patient groups

testicular tumours

Treatment:

Other: Analysis of methylation for the whole genome (sub-population of 15 samples from group 1)

Other: Analysis of methylation profiles of numerous GSI and TE

Other: Method of protein detection by immunostaining and flow cytometry

Other: Extraction genomic DNA

fertile

Treatment:

Other: Analysis of methylation profiles of numerous GSI and TE

Other: Method of protein detection by immunostaining and flow cytometry

Other: Extraction genomic DNA

infertile

Treatment:

Other: Analysis of methylation profiles of numerous GSI and TE

Other: Method of protein detection by immunostaining and flow cytometry

Other: Extraction genomic DNA