Epigenetic Reprogramming of Monocytes in Patients With Coronary Atherosclerosis

Radboud University Medical Center

Status

Completed

Conditions

Atherosclerosis

Study type

Observational

Funder types

Other

Identifiers

NCT02393768

SB EpiCard

Details and patient eligibility

About

In this study, the investigators will determine whether patients with documented atherosclerosis are characterized by specific epigenetic changes in circulating cells of the innate immune system, compared to patients without atherosclerosis.

Full description

Rationale: The innate immune system plays a pivotal role in the development and progression of atherosclerosis. Recently, it was reported that monocytes can develop a long-lasting immunological memory after stimulation with various microorganisms, which has been termed 'trained innate immunity'. This memory is induced by epigenetic reprogramming. We hypothesize that trained monocytes augment atherogenesis.

Objective: The main objective is to study whether patients with coronary atherosclerosis show specific epigenetic changes in the promoter regions of pro-inflammatory cytokines and chemokines and whether this correlates with the inflammatory phenotype of these cells.

Study design: Observational study

Study population: Adult patients who have had computed tomography coronary angiography because of chest pain: 20 patients without atherosclerosis and 20 patients with severe atherosclerosis will be included.

Enrollment

40 patients

Sex

All

Ages

18+ years old

Volunteers

No Healthy Volunteers

Inclusion criteria

Patients (male/female),
aged >18 years,
admitted to the acute coronary unit of the CWZ hospital for evaluation of chest pain.
No coronary atherosclerosis
Or Severe atherosclerosis

Exclusion criteria