ESWT on Human Gingival Fibroblasts

Dr Prabhuji MLV

Status: Completed

Conditions: Periodontal Attachment Loss

Treatments: Device: extracorporeal shockwave device; Other: Fibroblast cell lines not subjected to shockwaves from extracorporeal shockwave device

Study type: Interventional

Funder types: Other

Identifiers

NCT05766293

KCDS/48

Details and patient eligibility

About

The idea of treating different deformities or diseases in the maxillofacial region with Extracorporeal Shockwave Therapy (ESWT) has recently become popular. Shockwave therapy was first introduced in Germany in 1980s and has been widely used in medical practice for the management of urolithiasis, cholelithiasis and in head and neck region for sialolithiasis.

The present study 'Application of Extracorporeal Shock Wave Therapy on human gingival derived fibroblasts In-vitro study' was done to explore and evaluate the effect of shockwaves. Further, these cells were assessed for Cell - Cell interaction and Cell Viability.

In this in-vitro study, 20 fibroblast cells were included. 10 samples were considered for Control group and the remaining 10 samples were considered as Test group. Extracorporeal shockwave was applied to the Test samples. They were further analysed

Full description

BACKGROUND:

Improved understanding of human biology at the molecular level, scientific and technologic advances are emerging aspects in dentistry. The idea of treating different deformities or diseases in the maxillofacial region with Extracorporeal Shockwave Therapy (ESWT) has recently become popular. Shockwave therapy was first introduced in Germany in 1980s and has been widely used in medical practice for the management of urolithiasis, cholelithiasis and in head and neck region for sialolithiasis.

AIM:

The present study 'Application of Extracorporeal Shock Wave Therapy on human gingival tissue derived fibroblasts In-vitro study' was done to explore and evaluate the effect of shockwaves. Further, fibroblast cells were assessed for Cell - Cell interaction and Cell Viability.

MATERIALS AND METHODS:

In this in-vitro study, 20 fibroblast cells were included. 10 samples were considered for Control group and the remaining 10 samples were considered as Test group. Extracorporeal shockwave was applied to the Test samples. They were further analysed using wound scratch assay which was used to assess Cell proliferative rate and scratch closure through live imaging. MTT assay and ATP assay was used to determine Cell viability after exposure to shockwaves.

Enrollment: 20 patients

Sex: All

Volunteers: No Healthy Volunteers

Inclusion criteria

• Viable human gingival fibroblast
• Healthy gingival tissue

Exclusion criteria

• contaminated samples

Trial design

Primary purpose: Health Services Research

Allocation: Non-Randomized

Interventional model: Sequential Assignment

Masking: None (Open label)

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20 participants in 2 patient groups, including a placebo group

Shockwave treated group

Active Comparator group

Description:

Effect of Extracorporeal shockwaves on Fibroblast cell proliferation and cell viability

Treatment:

Device: extracorporeal shockwave device

No shockwave group

Placebo Comparator group

Description:

Fibroblast cell lines assessed for proliferative rate and viability without shockwave exposure.

Treatment:

Other: Fibroblast cell lines not subjected to shockwaves from extracorporeal shockwave device