Evaluate the Performance of Genetic Amplification by Polymerase Chain Reaction (PCR) and the "Mannan Antigenemia and Antimannan Antibodies Couple as a Means of Diagnosis and a Marker of Follow-up in Invasive Candidiasis. (EMPIRICAND)

University Hospital Center (CHU) Dijon Bourgogne

Status

Completed

Conditions

Invasive Candidiasis; Treatment With Echinocandin

Treatments

Biological: Blood sample taken on the day the treatment is initiated

Biological: Blood sample taken on day 3 after initiation of treatment

Biological: Blood sample taken on day 10 after initiation of treatment

Biological: Blood sample taken on day 7 after initiation of treatment

Biological: Blood sample taken on day 5 after initiation of

Study type

Observational

Funder types

Other

Identifiers

NCT02333448

Charles MSD 2014

Details and patient eligibility

About

The study consists in taking 4 tubes of blood at different times over a period of 10 days, via a catheter (central venous or arterial catheter) already in place in the usual therapeutic management. These samples will make it possible to measure blood levels of certain markers specific to invasive candidiasis. PCR will be used to quantify fungal load precisely, that is to say the quantity of yeast present in the blood and to monitor this quantity over time.

These samples will be transferred to a specialized unit and stored for a maximum of three years for use at the end of the study.

Enrollment

80 patients

Sex

All

Ages

18+ years old

Volunteers

No Healthy Volunteers

Inclusion criteria

Patient informed about the study
Patients aged at least 18 years admitted to a medical Intensive Care or general intensive care
Patients with suspected invasive candidiasis - that is to say:
Presenting persistent sepsis despite broad-spectrum antibiotherapy for at least 48 hours or targeted antibiotherapy for a documented bacterial infection (sepsis will be defined according to:
- the usual SIRS criteria, at least 2 of the following 4: temperature > 38°C or < 36°C, tachycardia > 90/min, respiratory rate > 20/min or blood pressure carbon dioxide (PaCO2 ) < 32 mmHg, leukocytosis > 12000/mm3 or < 4000/mm3 or > 10% of immature forms.
- Persistent hemodynamic instability (impossibility to significantly diminish catecholamine requirement, need for rapid vascular resuscitation > 1000 ml over the previous 24 hours)
Candida score ≥ 3 with multifocal colonization
Decision to initiate echinocandin therapy made by the clinician in charge of the patient