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Evaluation of Cell Membrane Expression of Annexin A2 on Monocytes by Flow Cytometry in Primary Antiphospholipid Syndrome (MONOCYTAN)

C

Centre Hospitalier Universitaire, Amiens

Status

Enrolling

Conditions

Antiphospholipid Syndrome

Treatments

Biological: blood withdrawal

Study type

Interventional

Funder types

Other

Identifiers

NCT06373926
PI2019_843_0010

Details and patient eligibility

About

Annexin A2 (ANXA2), an endothelial cell receptor for plasminogen and tissue plasminogen activator, plays a pivotal role in regulation of fibrinolysis in vitro and in vivo and has been identified as a new autoantigen in antiphospholipid syndrome (APS). ANXA2 can exist as a monomer or a heterotetrameric complex with S100A10 protein. The aim of this study was to evaluate the cell membrane expression of ANXA2 on circulating monocytes in APS by flow cytometry. Several pathogenic mechanisms are involved in APS such as activation of endothelial cells, platelets and monocytes, inhibition of the natural anticoagulant protein C/protein S pathway, activation of the complement system and also impairment of fibrinolysis. Annexin A2 which hits binding partner S100A10, ANXA2 forms a cell surface complex that regulates generation of plasmin. ANXA2 is involved in the pathogenesis of APS-associated through several possible mechanisms. Human peripheral blood monocytes represent the major circulating ANXA2-expressing cell and ANXA2-mediated assembly of plasminogen and tissue activator of plasminogen (tPA) on monocyte/macrophages contributes to plasmin generation. Thus the investigators could suppose that decrease of cell membrane expression of ANXA2 on circulating monocytes represent a new pathogenic mechanism in APS.

Enrollment

60 estimated patients

Sex

All

Ages

18+ years old

Volunteers

Accepts Healthy Volunteers

Inclusion criteria

  • APS patients who fulfilled the revised criteria for APS

Exclusion criteria

  • Patients less than 18 years old
  • Solid and hematological malignancies
  • Other autoimmune diseases
  • Cirrhosis

Trial design

Primary purpose

Diagnostic

Allocation

Non-Randomized

Interventional model

Parallel Assignment

Masking

None (Open label)

60 participants in 2 patient groups

case
Experimental group
Description:
APS patients
Treatment:
Biological: blood withdrawal
control
Active Comparator group
Description:
healthy individuals
Treatment:
Biological: blood withdrawal

Trial contacts and locations

1

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Central trial contact

Valéry Salle, MD

Data sourced from clinicaltrials.gov

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