Evaluation of Different Scaffolds in Regenerative Endodontic Treatment

British University In Egypt

Status: Completed

Conditions: Endodontic Disease; Regenerative Endodontics

Treatments: Procedure: Injectable platelet-rich fibrin (i-PRF) scaffold; Procedure: platelet-rich fibrin (PRF)scaffold; Procedure: blood clot (BC) scaffold

Study type: Interventional

Funder types: Other

Identifiers

NCT06945965

Regenerative Endodontic

Details and patient eligibility

About

The aim of this study is to evaluate and compare the regenerative potentials of Injectable Platelet-Rich Fibrin (I-PRF), Platelet-Rich Fibrin (PRF), and Blood Clot (BC) as different scaffolds regarding:

Radiographic outcomes (increase of root length, increase in thickness of dentinal wall, healing of periapical lesion, and apical closure).

Clinical outcomes in the treatment of young, immature permanent teeth with necrotic pulps.

Enrollment: 30 patients

Sex: All

Ages: 6 to 25 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Necrotic immature permanent anterior teeth.
• Teeth not indicated for post/core final restoration.
• Radiographically: The root has incomplete development with an apical opening of more than 1 mm.

Exclusion criteria

• Medically compromised patients with blood and immunity disorders. Family history of autoimmune disorder. Also, patients with physical or mental handicapping conditions.
• Teeth with questionable restorability of the remaining crown/root structure.
• Pathological tooth mobility.
• Radiographically: Evidence of root fracture or root resorption (external or internal).

Trial design

Primary purpose: Treatment

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: Double Blind

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30 participants in 3 patient groups

Regeneration with Injectable platelet-rich fibrin (i-PRF) scaffold

Experimental group

Description:

10 ml of venous blood was drawn from the patient's arm. Then it was collected in a sterile tube without anticoagulant. * The collected blood was centrifuged immediately. * The separated plasma and platelets form a light-yellow-colored layer, which is situated at the top of the tube. This is then aspirated using a syringe with a needle and amounts to a partially active injectable form.

Treatment:

Procedure: Injectable platelet-rich fibrin (i-PRF) scaffold

Regeneration with platelet-rich fibrin (PRF)scaffold

Experimental group

Description:

10 ml venous blood was drawn from the patient's arm * Then it was collected in a sterile tube without anticoagulant. * The collected blood was centrifuged immediately. * Following the centrifugation, the fibrin clot was formed in the middle of the tube between the red corpuscles at the bottom and acellular plasma at the top.

Treatment:

Procedure: platelet-rich fibrin (PRF)scaffold

Regeneration with blood clot (BC) scaffold

Active Comparator group

Description:

The file will be passed 2 mm beyond the apical end to induce bleeding inside the canal till approximately 2-3 mm below the CEJ.

Treatment:

Procedure: blood clot (BC) scaffold