Evaluation of Nucleotide-Binding Oligomerization Domain-Containing Protein (NOD) 1 and NOD 2

Medipol University

Status: Not yet enrolling

Conditions: Periodontal Diseases Inflammation

Treatments: Other: Saliva and Serum sampling

Study type: Interventional

Funder types: Other

Identifiers

NCT07160894

672

Details and patient eligibility

About

The host immune response to microorganisms is primarily recognized by pattern recognition receptors (PRRs), including toll-like receptors and nucleotide-binding oligomeric domain (NOD) receptors (NLRs). The aim of this study was to examine the relationship between periodontal disease and NOD in serum and saliva samples.

Full description

Healthy participants (n:25), gingivitis (n:25) patients and stage III, grade B periodontitis (n:25) patients enrolled for this study. Clinical periodontal parameters and salivary flow rate were noted. Serum and saliva samples were collected to measure NOD1, NOD2, IL-10 and IL-6 levels.

Enrollment: 75 estimated patients

Sex: All

Ages: 18 to 65 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• systemically healthy

  • clinical diagnosis of periodontal health
  • clinical diagnosis of periodontitis
  • clinical diagnosis of gingivitis

Exclusion criteria

• • history of regular use of systemic antibiotics anti-inflammatory, or antioxidant drugs (previous 3 months)

  • nonsurgical periodontal treatment (previous 6 months)
  • surgical periodontal treatment (previous 12 months)
  • presence of<10 teeth
  • current medications affecting gingival health (calcium channel blockers, phenytoin, cyclosporine, and hormone replacement therapy)
  • diabetes
  • diagnosis of rheumatoid arthritis
  • pregnancy
  • lactating
  • smoking
  • excessive alcohol consumption.

Trial design

Primary purpose: Prevention

Allocation: Randomized

Interventional model: Single Group Assignment

Masking: None (Open label)

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75 participants in 2 patient groups

Active Comparator

Active Comparator group

Description:

Collection of patients' saliva and serum and analysis of sample molecules.

Treatment:

Other: Saliva and Serum sampling

Other: Saliva and Serum sampling

Experimental

Experimental group

Description:

ELISA method will be used to evaluate NOD1 and NOD2, IL-10, IL-6 molecules in the saliva and serum samples obtained.

Treatment:

Other: Saliva and Serum sampling

Other: Saliva and Serum sampling