EVALUATION OF SALIVA AND SERUM HEME OXYGENASE, ARYLESTERASE AND NUCLEAR FACTOR ERYTHROID 2-RELATED FACTOR 2 LEVELS IN PATIENTS WITH STAGE III PERIODONTITIS

Saglik Bilimleri Universitesi

Status: Completed

Conditions: Periodontitis Stage III; Periodontal Health

Treatments: Diagnostic Test: Gingival Index; Diagnostic Test: Salivary Nuclear Factor Erythroid 2 Related Factor 2; Diagnostic Test: Serum Total Oxidant Status; Diagnostic Test: Salivary Oxidative Stress Index; Diagnostic Test: Clinical Attachment Loss; Diagnostic Test: Serum Nuclear Factor Erythroid 2 Related Factor 2; Diagnostic Test: Serum Total Antioxidant Status; Diagnostic Test: Pocket Deep; Diagnostic Test: Serum Arylesterase; Diagnostic Test: Bleeding on Probing Index; Diagnostic Test: Salivary Arylesterase; Diagnostic Test: Plaque Index; Diagnostic Test: Salivary Heme Oxygenase; Diagnostic Test: Serum Heme Oxygenase; Diagnostic Test: Salivary Total Oxidant Status; Diagnostic Test: Serum Oxidative Stress Index; Diagnostic Test: Salivary Total Antioxidant Status

Study type: Observational

Funder types: Other

Identifiers

NCT06986044

GülhaneDiş

Details and patient eligibility

About

Periodontal disease is a chronic and progressive inflammatory disease in which the hard and soft tissues that support the teeth are damaged. It is caused by the interaction between harmful bacteria and the body's immune responses, and most periodontal tissues are damaged by the body's abnormal response to these microorganisms and their products. When bacteria enter the body, immune cells (neutrophils) produce reactive oxygen species (ROS) in a process called the "respiratory burst". These ROS damage cells, causing tissue destruction through a variety of mechanisms, including DNA damage, fat oxidation and protein damage. Studies have shown that neutrophils from individuals with periodontal disease produce more ROS than neutrophils from healthy individuals. High amounts of ROS lead to oxidative damage to gum tissue, periodontal ligament and alveolar bone. Oxidative stress occurs when antioxidants in the body are insufficient or when high levels of ROS are present. Therefore, disruption of the balance between oxidant and antioxidant activities is considered an important cause of oxidative damage in periodontal tissues. Parameters such as total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) are used to determine oxidative stress.

Furthermore, some enzymes such as arylesterase (ARE), heme oxygenase (HO) and nuclear factor erythroid 2-related factor 2 (NRF-2) are involved in defense mechanisms against oxidative stress. Many recent studies have shown a strong association between oxidative stress and periodontal disease.

Full description

This study was divided into 2 groups, 37 systemically and periodontally health individuals and 37 systemically healthy individuals with stage III grade B periodontitis were included. Clinical periodontal parameters plaque index (PI), gingival index (GI), probing pocket depth (SCD), bleeding on probing (BOP) and clinical attachment level (CAL) were recorded and saliva and serum samples were collected. ELISA method was used for analyses of TOS, TAS, OSI, ARE, HO-1, NRF-2 levels.

Enrollment: 74 patients

Sex: All

Ages: 18 to 65 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• systemically healthy
• between the ages of 18-65

Exclusion criteria

• Patients with systemic diseases and/or conditions that may affect periodontal tissues (cardiovascular diseases, immune system diseases, rheumatoid arthritis, diabetes mellitus, chemotherapy/radiotherapy).
• taking any medication that may have an effect on periodontal tissues,
• less than 20 natural teeth excluding third molars
• used antibiotics, anti-inflammatory or immunosuppressive drugs
• periodontal treatment in the last 6 months
• smokers
• pregnant or lactating women.

Trial design

74 participants in 2 patient groups

stage III grade B periodontitis

Description:

Pocket deep ≤ 6 mm, clinical attachment loss ≥ 5 mm, tooth loss related to periodontitis ≤ 4, radiographic bone loss/age between 0.25-1 and periodontal tissue destruction compatible with biofilm accumulation.

Treatment:

Diagnostic Test: Salivary Total Antioxidant Status

Diagnostic Test: Serum Oxidative Stress Index

Diagnostic Test: Serum Heme Oxygenase

Diagnostic Test: Salivary Total Oxidant Status

Diagnostic Test: Plaque Index

Diagnostic Test: Salivary Heme Oxygenase

Diagnostic Test: Salivary Arylesterase

Diagnostic Test: Bleeding on Probing Index

Diagnostic Test: Serum Arylesterase

Diagnostic Test: Pocket Deep

Diagnostic Test: Serum Nuclear Factor Erythroid 2 Related Factor 2

Diagnostic Test: Serum Total Antioxidant Status

Diagnostic Test: Clinical Attachment Loss

Diagnostic Test: Salivary Oxidative Stress Index

Diagnostic Test: Salivary Nuclear Factor Erythroid 2 Related Factor 2

Diagnostic Test: Serum Total Oxidant Status

Diagnostic Test: Gingival Index

periodontally health

Description:

Pocket deep ≤ 3 mm, bleeding on probing index ≤ %10 and gingival index ≤ 1

Treatment:

Diagnostic Test: Salivary Total Antioxidant Status

Diagnostic Test: Serum Oxidative Stress Index

Diagnostic Test: Serum Heme Oxygenase

Diagnostic Test: Salivary Total Oxidant Status

Diagnostic Test: Plaque Index

Diagnostic Test: Salivary Heme Oxygenase

Diagnostic Test: Salivary Arylesterase

Diagnostic Test: Bleeding on Probing Index

Diagnostic Test: Serum Arylesterase

Diagnostic Test: Pocket Deep

Diagnostic Test: Serum Nuclear Factor Erythroid 2 Related Factor 2

Diagnostic Test: Serum Total Antioxidant Status

Diagnostic Test: Clinical Attachment Loss

Diagnostic Test: Salivary Oxidative Stress Index

Diagnostic Test: Salivary Nuclear Factor Erythroid 2 Related Factor 2

Diagnostic Test: Serum Total Oxidant Status

Diagnostic Test: Gingival Index