Evaluation of Viral Replication by Tonate Virus (TONV) and Zika Virus (ZIKV), Within an ex Vivo Trophoblast and Placental Model (ZITOPEx)

Assistance Publique - Hôpitaux de Paris

Status: Not yet enrolling

Conditions: Zika Virus Infection

Treatments: Biological: Cell line and virus; Biological: Infection of human placental culture explants; Biological: Determination of viral load in tissues by qRT-PCR

Study type: Observational

Funder types: Other

Identifiers

NCT05589012

2020-A01318-31

Details and patient eligibility

About

Prospective, non-interventional study carried out after culturing placental trophoblastic tissue ex vivo and infection with Zika and Tonate

Full description

The analysis will relate for each virus studied + control + favipiravir to 1 placenta in the 1st trimester and 1 placenta at term.

2 experiments per virus will be planned.

Enrollment: 8 estimated patients

Sex: Female

Ages: 18+ years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Patient over 18 years old
• Singleton pregnancy of normal course, at term, birth by caesarean section OR
• Trophoblast from Voluntary Termination of Pregnancy (IVG), after endo-uterine aspiration

Exclusion criteria

• Need for pathological, genetic or bacteriological examination of the placenta
• Multiple pregnancy
• HBV+ (Hepatitis B positive), HCV+ (Hepatitis C positive) , known CMV seroconversion during pregnancy (CytoMégaloVirus)
• Immunosuppression (drugs, corticosteroids, etc.)
• Diabetes
• Pre eclampsia
• Intrauterine growth retardation (IUGR),
• Vascular or placental pathology.
• Refusal to participate
• Patient under guardianship / curatorship / security measure
• Patient under AME (state medical aid)

Trial design

8 participants in 1 patient group

Human placentas and trophoblasts (biological waste) from uncomplicated pregnancies.

Description:

The placentas will be collected following a birth by caesarean section after oral and written information by delivery of the information note for women who have had a normal singleton pregnancy at term. Similarly, trophoblasts are collected following endo-uterine aspiration in the context of voluntary termination of pregnancy. * Cell line and virus * Infection of human placental culture explants * Determination of viral load in tissues by qRT-PCR

Treatment:

Biological: Determination of viral load in tissues by qRT-PCR

Biological: Infection of human placental culture explants

Biological: Cell line and virus