Evaluation the Association of Microbiome Between Respiratory Tract Samples and Stool Samples in Pneumonic Patients Accompanied by Respiratory Failure

Taichung Veterans General Hospital

Status: Completed

Conditions: Respiratory Failure; Lower Resp Tract Infection; Pneumonia, Bacterial; Microbiota

Treatments: Diagnostic Test: 16s RNA sequence of sputum, bronchoalveolar lavage fluid (options), stool

Study type: Observational

Funder types: Other

Identifiers

NCT03379779

IGA-10601

Details and patient eligibility

About

A prospective observational study. Enrolled participants admitted to ICU due to pneumonia and respiratory failure need mechanical ventilator support. Investigators collected the residual specimens, such as sputum from endotrachea aspiration, bronchoalveolar lavage fluid in those participants as the usual care in the ICU. Those residual samples were sent to extract RNA and sequence by using high-throughput sequencing (next-generation sequencing) method.

Investigators will compared the microbiome feature between lower respiratory tract and stool specimens in those participants diagnosed as pneumonia with respiratory failure.

Enrollment: 200 patients

Sex: All

Ages: 40+ years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Adults aged than 40 years old
• Diagnosed as pneumonia
• Respiratory failure with mechanical ventilator support

Exclusion criteria

• Less than 40 years old
• Has been treated with antibiotics more than 7 days within two weeks before enrolling into study
• Suspected or active pulmonary tuberculosis infection

Trial design

200 participants in 1 patient group

Pneumonia patient with respiratory failure

Description:

Sputum and stool sampling day 1, 3 and 7 after enrolling into study

Treatment:

Diagnostic Test: 16s RNA sequence of sputum, bronchoalveolar lavage fluid (options), stool