Exercise-induced Changes in Exosomes

University of California (UC) Davis

Status: Completed

Conditions: Exosomes; Exercise; Connective Tissue

Treatments: Procedure: Resistance Exercise

Study type: Interventional

Funder types: Other

Identifiers

NCT05616234

1956959

Details and patient eligibility

About

The proposed study aims to shed light on the function/importance/relevance of exercise-induced changes in exosomes to connective tissues. Exosomes are known to increase robustly in response to exercise. We have previously shown that serum isolated from subjects after they lift heavy weights increases human engineered ligament collagen content and mechanics more than serum from before they lift weight. Further, we showed that exercise-induced changes in hormones could not explain the change in ligament structure or function. These data indicate that there is a significant gap in our understanding of muscle-connective tissue crosstalk. To address this gap, the current proposal seeks to: i) isolate and sequence exosomal RNA (long non-coding, miR, and mRNA) and ii) determine whether exosomes isolated from serum after exercise increase engineered ligament mechanics and collagen content.

Enrollment: 12 patients

Sex: All

Ages: 18 to 35 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Young healthy adults (18-35 y)
• Experience with exercise (>1y)

Exclusion criteria

• Pregnancy
• Smoking
• Receiving any medication that may interfere with the study outcomes

Trial design

Primary purpose: Treatment

Allocation: N/A

Interventional model: Single Group Assignment

Masking: None (Open label)

---

12 participants in 1 patient group

Exercise trial

Experimental group

Description:

Participants will perform a bout of exercise. In addition, a baseline and post-exercise blood sample will be drawn.

Treatment:

Procedure: Resistance Exercise