Exhaled Breath Condensate Analysis in Mechanically Ventilated Patients

T

The Institute of Molecular and Translational Medicine, Czech Republic

Status

Not yet enrolling

Conditions

Critical Illness
Acute Respiratory Failure

Treatments

Diagnostic Test: Proteomic analysis of exhaled breath condensate

Study type

Observational

Funder types

Other

Identifiers

NCT06189924
64734

Details and patient eligibility

About

Mechanically ventilated intensive care patients will be sampled for a small amount of exhaled breath condensate from the ventilator circuit and for venous blood. Proteomic analysis of the exhaled breath condensate will be performed using mass spectrometry and in the blood sample, corresponding changes in the DNA, RNA, proteins, and metabolites will be studied. Resulting profiles will be correlated with routinely monitored parameters in order to identify patterns corresponding to various pathologies in order to enable their early detection.

Full description

Exhaled breath condensate (EBC) is a product of gas exchange in the lungs. It contains plethora of substances of possible diagnostic value and its composition is likely to change rapidly in response to the development of pathological conditions, especially in the lungs. Mechanically ventilated intensive care patients with various pathological conditions will be sampled for a small amount of EBC from the ventilator circuit and for venous blood. A sampling method not affecting relevant parameters of mechanical ventilation will be developed and standardised. EBC samples will be frozen and after processing, proteomic analyses will be performed using mass spectrometry. In the blood sample, corresponding changes in the DNA, RNA, proteins, and metabolites will be studied using molecular biological methods. Resulting profiles will be correlated with routinely monitored parameters (such as inflammation markers, microbiological findings and mechanical ventilation parameters) with the aim of finding patterns corresponding to the early stages of various pathological conditions focusing on lung tissue. Identification of patterns specific to initial stages of lung and other organ pathologies would enable their early treatment possibly shortening mechanical ventilation/ICU stay and reducing morbidity/mortality.

Enrollment

200 estimated patients

Sex

All

Ages

18+ years old

Volunteers

No Healthy Volunteers

Inclusion criteria

Intensive care setting Mechanical ventilation Ventilator/ventilator circuit technically suitable for sampling of exhaled breath condensate

Exclusion criteria

Mechanical ventilation challenging to the extent that the insertion of the sampling kit into the ventilator circuit would substantially increase the risk for respiratory deterioration

Trial design

200 participants in 2 patient groups

Mechanically ventilated patients with primary or secondary lung injury
Description:
Mechanically ventilated patients with primary or secondary lung injury due to various pathologies. Patients will be treated at intensive care units (ICU).
Treatment:
Diagnostic Test: Proteomic analysis of exhaled breath condensate
Mechanically ventilated patients without lung injury
Description:
Mechanically ventilated patients without lung injury, with various pathologies. Patients will be treated at intensive care units (ICU).
Treatment:
Diagnostic Test: Proteomic analysis of exhaled breath condensate

Trial contacts and locations

2

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Central trial contact

Emil Berta, MD PhD; Karel Axmann, MD

Data sourced from clinicaltrials.gov

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