Expression of miRNa-138 and Cyclin D1 in Oral Mucosa of Patients With Oral Lichen Planus

Cairo University (CU)

Status: Completed

Conditions: Oral Lichen Planus

Study type: Observational

Funder types: Other

Identifiers

NCT02834520

MiRNA-138_cyclin D1_OLP

Details and patient eligibility

About

60 individuals were subdivided into 2 groups, 30 patients with oral lichen planus, 30 control individuals. Expression of miRNA-138 and cyclin D1 were evaluated in oral mucosa utilizing Quantitative real-time polymerase chain reaction and immunohistochemistry.

Full description

60 individuals were subdivided into 3 groups, into 2 groups, 30 patients with oral lichen planus, 30 control individuals having age, gender and periodontal status matched with both patients groups acting as a control group. Relative gene expression of miRNA-138 was estimated using Quantitative real-time polymerase chain reaction technique in oral mucosa of both oral lichen planus patients and control subjects. Sub grouping of oral lichen planus patients were performed into reticular, atrophic and erosive groups (n=10 each). Cyclin D1 gene expression was estimated using Quantitative real-time polymerase chain reaction technique and Cyclin D1 protein level was estimated using immunohistochemistry.

Enrollment: 60 patients

Sex: All

Ages: 45 to 65 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• patients with oral lichen planus.

Exclusion criteria

• any systemic disease other than oral lichen planus.
• smokers.
• pregnant females.
• periodontal diseases.

Trial design

60 participants in 2 patient groups

oral lichen planus

Description:

30 patients suffering from reticular, erosive and atrophic oral lichen planus

control subjects

Description:

30 individuals age, gender and periodontal status matched with oral lichen planus patients and not suffering from any oral mucosal lesions or periodontal disease.