Expression of Toll-Like Receptors 2 and 4 in Asthmatic Patients.

Fundació Institut de Recerca de l'Hospital de la Santa Creu i Sant Pau

Status

Completed

Conditions

Asthma

Treatments

Other: TLRs

Study type

Interventional

Funder types

Other

Identifiers

NCT02028637

43/2009

Details and patient eligibility

About

Introduction: Recently it has involved the role of TLR (toll-like receptors) in the pathogenesis of asthma. TLRs are a family of proteins responsible for the recognition of Pathogen-Associated Molecular Patters (PAMPs). The possible role that TLRs play in IgE-dependent asthma (not eosinophilic) and its recognition as a new endotype asthma, could open new therapeutic expectations.

Objective: To analyze the expression of TLR 2 and 4 in monocytes/ macrophages and neutrophils in peripheral blood and induced sputum from asthmatic patients

Full description

The investigators studied patients with asthma. All patients underwent the same day an induced sputum, pulmonary function studies, fractional exhaled nitric oxide, blood total IgE and skin test by prick technique. Asthmatic patients were classified according to the criteria of the GINA 2012. The cellular composition was analyzed by flow cytometry. Expression of TLR2 and TLR4 were analyzed using monoclonal antibodies conjugated with fluorochromes appropriate.

Enrollment

59 patients

Sex

All

Ages

18 to 80 years old

Volunteers

No Healthy Volunteers

Inclusion criteria

Adults with persistent asthma (defined as per the Global INitiative for Asthma Management (GINA) criteria)

Exclusion criteria

If they had a respiratory tract infection or asthma exacerbation within 30 days prior to inclusion
If they were receiving oral steroids or immunosuppressive treatments.