Expression Profiling of microRNA Following Administration of Dexmedetomidine

First Affiliated Hospital of Kunming Medical University

Status: Completed

Conditions: Coronary Disease

Treatments: Drug: Dexmedetomidine Injection

Study type: Interventional

Funder types: Other

Identifiers

NCT03213743

1stKunmingMCQJQ

Details and patient eligibility

About

The purpose of the study is to find out the differential expression profiling of microRNA before and after adiministration of dexmedetomidine in patients undergoing procedures, and then investigators will do some in vitro studies to validate the functions of the microRNA.

Full description

Background and objective: The use of dexmedetomidine may have benefits on the clinical outcomes of cardiac surgery. MicroRNAs are non-coding RNA sequences that act as regulators of gene expression. Investigators conduct a study to determine the role of microRNAs in cardioprotection mediated by dexmedetomidine.

Methods: In this study 3 patients were selected. The blood sample were taken before administration of dexmedetomidine.Investigators infused loading dose of dexmedetomidine for 10 minutes and maintaining dese of dexmedetomidine for 20 minutes.Thirty minutes after the administration, another blood samples were taken and stored in the liquid nitrogen and both the samples were marked. Those sample were analyzed for differentially expressed microRNA with Exiqon miRNA Array.

Enrollment: 3 patients

Sex: All

Ages: 40 to 70 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• The patients without arrithmia will undergo procedure.

Exclusion criteria

• The patients have bradycardia (hear rate is less than 60 beats per minute) or conduction block.

Trial design

Primary purpose: Basic Science

Allocation: N/A

Interventional model: Single Group Assignment

Masking: None (Open label)

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3 participants in 1 patient group

before dexmedetomidine

Other group

Description:

We took blood samples before the administration of dexmedetomidine to determine exression level of microRNA in the subjects.

Treatment:

Drug: Dexmedetomidine Injection