Ferroptosis Study in SF3B1-mutant Myelodysplastic Syndromes (FerMDS)

University Hospital of Bordeaux

Status: Enrolling

Conditions: Myelodysplastic Syndromes; Ferroptosis

Treatments: Biological: Biological sampling

Study type: Interventional

Funder types: Other

Identifiers

NCT05924074

CHUBX 2022/43

Details and patient eligibility

About

Myelodysplastic syndromes (MDS) are clonal diseases of hematopoietic stem cells (HSC) characterized by dysplastic and inefficient hematopoiesis related to excessive progenitor cell death. Ferroptosis is a recently described cell death mechanism and we think that it could be a major player in the pathophysiology of MDS, involved in the cell death that characterizes these diseases and contributing to cytopenias. The study aims to demonstrate that there is a significant activation of this phenomenon in MDS patients compared to a population of subjects without MDS.

Full description

Myelodysplastic syndromes (MDS) are hematological malignancies characterized by a defect in blood cells production. Their pathophysiology remains poorly understood, but an excessive death of progenitor cells is considered as a key mechanism contributing to the appearance of cytopenia. Furthermore, it is known that there are abnormalities of iron metabolism in MDS, especially in patients with ring sideroblasts and SF3B1 mutation. The classical therapeutic strategy in MDS relies on symptomatic management of cytopenias (transfusions, growth factors) associated with demethylating agents in high-risk forms. Unfortunately, these treatments only stabilize the disease and only allogeneic bone marrow transplantation (reserved to limited number of patients) can cure the patients. Therefore, there is a urgent need to identify new therapeutic targets in these diseases.

An excessive apoptosis activation has been shown in MDS for a long time. However, other cell death pathways could also contribute to their pathophysiology. Among them, ferroptosis, a cell death process triggered by the accumulation of free iron in the cell, seems to be a promising candidate.

The project is proposed to study ferroptosis in SF3B1-mutant MDS patients. An additionnal bone marrow sample will be aspirate at diagnosis. Ferroptosis will be analyzed using flow cytometry (labeling of peroxidized lipids with C11-BODIPY). The percentage of cells in ferroptosis will be compared between SF3B1-mutant MDS patients and control patients (patients evaluated for Monoclonal Gammapathy of Unknown Significance-MGUS). The presence of an excess of ferroptosis in SF3B1-mutant MDS patients will be correlated to clinico-biological parameters. No follow up will be be performed.

Enrollment: 80 estimated patients

Sex: All

Ages: 18+ years old

Volunteers: No Healthy Volunteers

Inclusion criteria

For all :

• Patients of legal age (age ≥ 18 years)
• Subjects affiliated to or benefiting from a social security scheme
• Free, written and informed consent signed by the participant and the investigator

For MDS patients :

• Sampling at diagnosis for MDS patients (WHO 2016 criteria)
• Presence of ring sideroblasts on bone marrow smear

For MGUS patients :

• Sampling as part of the exploration of monoclonal gammopathy of undetermined significance (MGUS) for controls (WHO 2016 criteria).

Exclusion criteria

For all

• Patient transfused with red blood cells within 120 days prior to collection
• Patients treated with haematopoietic growth factors (EPO, TPO, G-CSF) within 30 days prior to collection
• Patients with conditions that affect systemic iron metabolism: hemochromatosis, Gaucher disease, ferroportin disease, porphyria cutanea tarda
• Person under a legal protection measure (legal protection, guardianship or curatorship)
• Person deprived of liberty by judicial or administrative decision
• Person who is unable to give consent
• Subject who is in an exclusion period after another study or who has participated in another interventional drug study within 30 days prior to entry into the protocol

Trial design

Primary purpose: Diagnostic

Allocation: Non-Randomized

Interventional model: Parallel Assignment

Masking: None (Open label)

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80 participants in 2 patient groups

SF3B1 mutant Myelodysplastic syndromes patients (MDS)

Experimental group

Description:

Patients diagnosed with MDS carrying the SF3B1 somatic mutation associated myelodysplastic neoplasm with ring sideroblasts

Treatment:

Biological: Biological sampling

Monoclonal Gammapathy of Unknown Significance patients (MGUS)

Active Comparator group

Description:

MGUS patients, referred to as normal bone marrow controls

Treatment:

Biological: Biological sampling