Fetuin-A Phosphorylation Status in Insulin Resistance and Metabolic Syndrome

Auburn University

Status: Completed

Conditions: Obesity; Metabolic Syndrome

Treatments: Other: Weight loss

Study type: Interventional

Funder types: Other

Identifiers

NCT03478046

07-210-MR0710

Details and patient eligibility

About

Fetuin-A has been identified as a novel physiological regulator of insulin action in vitro, in intact cells and in vivo in animals. Previous research has shown that circulating levels of fetuin-A were increased in animal models of insulin resistance and diabetes. Additionally, several human investigation studies demonstrate a correlation of fetuin-A levels with body mass index, insulin resistance, and a fatty liver. Recently, the investigators have elucidated the role of fetuin-A phosphorylation in the regulation of insulin action, demonstrating that phosphorylation is critical for the inhibitory activity of fetuin-A. The objectives of this study are twofold: (1) Quantitate phosphorylated fetuin-A levels in individuals with insulin resistance and metabolic syndrome, and (2) Investigate the effects of lifestyle modifications (acute or chronic exercise and dietary modifications) on fetuin-A phosphorylation and insulin sensitivity.

Full description

There are several objectives of this study: (1) Quantitate phosphorylated fetuin-A levels and the daily variation in these levels in individuals with insulin resistance and metabolic syndrome; (2) Investigate the short-term responses of fetuin-A phosphorylation and changes in insulin resistance that occur with a single session of aerobic exercise; (3) Characterize and compare the total and temporal changes in fetuin-A phosphorylation and insulin resistance that occur with an 8 to 10% weight loss induced by chronic exercise training and dietary modification, and; (4) Investigate the influence of weight loss on the short-term responses of fetuin-A phosphorylation and changes in insulin resistance that occur with a single session of aerobic exercise. Our hypothesis is that phosphofetuin-A levels are tightly correlated with insulin resistance and that lifestyle modifications will improve insulin sensitivity and decrease phosphorylated fetuin-A levels.

Enrollment: 42 patients

Sex: Male

Ages: 30 to 65 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• 30 to 65 years of age
• obese (BMI > 30 kg⋅m2 or % fat > 30, and waist girth > 88 cm)
• weight stable over the previous 6 months

Exclusion criteria

• smoker
• documented cardiovascular or metabolic disease
• currently taking medication to alter lipid or glucose metabolism
• practicing regular leisure physical activity over the previous 6 months
• engaged in strenuous vocational activity over the previous 6 months
• signs or symptoms of latent heart disease
• conditions that would preclude treadmill walking

Trial design

Primary purpose: Basic Science

Allocation: N/A

Interventional model: Single Group Assignment

Masking: None (Open label)

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42 participants in 1 patient group

Obese individuals

Experimental group

Description:

Apparently healthy, weight-stable, obese and physically inactive male volunteers will be recruited. Intervention is an 8 to 10% weight loss induced by chronic exercise training and dietary modification

Treatment:

Other: Weight loss