Fluorodeoxyglucose Positron Emission Tomography (FDG PET) Findings in Patients With Phenylketonuria Before and After KUVAN Therapy (PKU)

Children's Hospital of Philadelphia (CHOP)

Status

Completed

Conditions

Phenylketonuria

Treatments

Drug: Sapropterin

Study type

Interventional

Funder types

Other

Identifiers

NCT00986973

IRB 09-007154

Details and patient eligibility

About

The aim of this pilot study is to determine if there are any changes in brain glucose metabolism in the gray matter of patients with Phenylketonuria (PKU) and whether administration of Sapropterin (KUVAN) therapy can improve such deficits.

Full description

Phenylketonuria (PKU) is an autosomal recessive disorder resulting from a deficiency of phenylalanine hydroxylase, which converts phenylalanine to tyrosine. Phenylalanine hydroxylase is one of the three aromatic amino acid hydroxylases that utilizes tetrahydrobiopterin (BH4) as cofactor. The published reports indicate that there is altered energy metabolism in the brain of patients with PKU. Phenylalanine and its metabolites appear to impair several aspects of brain energetics including: (1) Inhibition of glucose uptake; (2) diminished glycosylation of cytoskeletal proteins; (3) Inhibition of pyruvate kinase; and (4) reduced flux through the glycolysis. Studies in vivo with magnetic resonance spectroscopy have demonstrated phenylalanine-responsive abnormalities in cerebral energy metabolism.

Positron Emission Tomography (PET) scanning with fluorodeoxyglucose (FDG-PET) is a non-invasive method that measures regional glucose metabolic rate with high resolution and absolute quantitation. To date this technology has been used only for single case reports or the investigation of white matter abnormalities in small numbers of patients with PKU.

The aim of this pilot study is to determine if there are any changes in brain glucose metabolism in the gray matter of patients with PKU and whether Sapropterin (KUVAN) can improve such deficits. This study will also elucidate the relationship between hyperphenylalaninemia, phenylalanine intake in diet, altered brain glucose handling and the neurocognitive profile of the patients with PKU before and after KUVAN therapy.

Enrollment

6 patients

Sex

All

Ages

18 to 50 years old

Volunteers

No Healthy Volunteers

Inclusion criteria

Males or females over the age of 18 years
Subject must be able to give independent informed consent
Girls must have a negative urine pregnancy test and must use an acceptable method of contraception, including abstinence, a barrier method (diaphragm or condom), Depo-Provera, or an oral contraceptive, for the duration of the study.
Subject must have a confirmed diagnosis of PKU
Subjects with Phenylalanine (Phe) levels over 10 mg/dL
Subjects naïve to KUVAN therapy or has not received KUVAN in the 6 months before screening

Exclusion criteria

Pregnancy
Cognitive deficits resulting from physical trauma (e.g. subject with history of severe birth trauma).
Neurologic comorbidities including a history of a stroke or a seizure disorder.
Laboratory abnormalities that indicate clinically significant hepatic disease Aspartate aminotransferase (AST)> 2.0 times the upper limit of normal, Alanine transaminase (ALT) > 2.0 times the upper limit of normal, Prothrombin Time (PT) > 2.0 times the upper limit of normal, Partial Thromboplastin Time(PTT) > 2.0 times the upper limit of normal
Subjects using medications such as steroids, insulin and glucagons that may interfere with the results of PET scan.
Subjects using medications that inhibit folate metabolism such as methotrexate
Subjects using medications known to affect nitric oxide-mediated vasorelaxation.
Subjects using Levodopa
Treatment with KUVAN in the past 6 months before study entry.
Treatment with any investigational product in the last 90 days before study entry