Forearm Immobilization, Metabolic Health, and Nutrition

University of Exeter

Status: Completed

Conditions: Healthy

Treatments: Behavioral: Forearm immobilization

Study type: Interventional

Funder types: Other

Identifiers

NCT04626401

200617

Details and patient eligibility

About

The present study will investigate the impact of dietary branched chain amino acid restriction on muscle atrophy, insulin sensitivity and muscle protein synthesis following short-term forearm immobilization

Full description

Twenty-four healthy young volunteers will undergo 2 days of forearm immobilization in combination with either a branched chain amino acid restricted diet or a control diet. Before and after immobilization, they will receive a stable isotope tracer infusion (5.5 h) combined with repeated blood and muscle sampling under insulin clamp conditions, in order to measure insulin sensitivity and muscle protein synthesis in the fasted and fed state.

Enrollment: 24 patients

Sex: All

Ages: 18 to 40 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Males and females 18-40 years of age
• Body mass index between 18.5 and 30 kg/m2

Exclusion criteria

• Smoking
• Any diagnosed metabolic impairment (e.g. type 1 or 2 diabetes)
• Any diagnosed cardiovascular disease
• Hypertension (≥140 mmHg systolic and/or ≥90 mmHg diastolic)
• Chronic use of any prescribed or over the counter pharmaceuticals (excluding oral contraceptives and contraceptive devices)
• Regular use of nutritional supplements
• Currently involved in a structured progressive resistance training programme (>3 times per week)
• A personal or family history of thrombosis, epilepsy, seizures or schizophrenia
• Any previous motor disorders or disorders in muscle and/or lipid metabolism
• Known hypokalaemia
• Presence of an ulcer in the stomach or gut and/or strong history of indigestion
• Known severe kidney problems
• Pregnant or breastfeeding
• Unable to give consent

Trial design

Primary purpose: Basic Science

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: Double Blind

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24 participants in 2 patient groups, including a placebo group

Control diet during immobilisation

Placebo Comparator group

Description:

A 2 day controlled dietary intervention.

Treatment:

Behavioral: Forearm immobilization

Branched chain amino acid restricted diet during immobilisation

Experimental group

Description:

A 2 day branched chain amino acid restricted dietary intervention.

Treatment:

Behavioral: Forearm immobilization