Free Amino-acid Composition of Peri-implant and Periodontal Conditions (AAs)

Medipol Health Group

Status: Completed

Conditions: Peri Implant Mucositis; Peri Implantitis; Peri-Implant Health

Treatments: Other: Saliva sampling; Other: Amino Acid Analysis

Study type: Observational

Funder types: Other

Identifiers

NCT07187323

645

Istanbul Medipol University

Details and patient eligibility

About

Peri-implant diseases, encompassing peri-implant mucositis and peri-implantitis, are biologically driven inflammatory conditions that threaten the long-term success of dental implants. The microbial signatures and their metabolic products have prompted growing interest in salivary biomarkers, particularly amino acids (AAs), which may reflect both microbial activity and host response.However, despite increasing evidence of periodontal disease, the role of salivary AAs in peri-implant conditions remains underexplored.The aim of this study is to comprehensively evaluate and compare the salivary free amino acid profiles among individuals with peri-implantitis, peri-implant mucositis, and healthy peri-implant mucosa, as well as those with periodontitis, gingivitis, and a healthy periodontium, and seeks to investigate whether distinct salivary amino acid signatures are linked to different stages and types of peri-implant and periodontal inflammation, and to evaluate their potential as non-invasive biomarkers for disease differentiation, activity, and severity

Full description

The study included one-hundred and thirty-two patients who applied to the Department of Periodontology at Istanbul Medipol University, Faculty of Dentistry. Four of the one-hundred and thirty-two patients declined to participate in the trial. Two patients were eliminated because they had recently taken antibiotics, six patients were discarded because they had recently received periodontal therapy, and six patients were excluded because they had systemic diseases, resulting in a final sample size of one-hundred and twenty patients .Quantitative analysis of amino acids in saliva was carried out using liquid chromatography-tandem mass spectrometry (LC-MS/MS) with a Thermo Scientific TSQ Quantum Access MAX system.

Enrollment: 120 patients

Sex: All

Ages: 20 to 65 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• be between 18 and 65 years of age
• have a minimum of 20 natural teeth, excluding third molars (for non-implant-treated groups)
• be edentulous patients treated with implants loaded at least 1 year prior (for implant-treated groups)
• be in a systemically healthy condition.

Exclusion criteria

• current tobacco use
• chronic use of any systemic medication
• use of antibiotics, anti-inflammatory drugs (including corticosteroids and NSAIDs), immunosuppressants, beta-blockers, calcium channel blockers, anticoagulants, or hormonal contraceptives within the 3 months prior to the study
• receipt of periodontal therapy within the past 6 months
• pregnancy or breastfeeding
• presence of orthodontic appliances a DMFT index (Decayed, Missing, and Filled Teeth per tooth) greater than 2

Trial design

120 participants in 2 patient groups

Saliva collection

Description:

Saliva and serum sampling Saliva were collected to analyze the selected markers as unstimulated samples during the early hours of the day. The saliva was centrifuged and then transferred into Eppendorf tubes. Venous puncture was performed after saliva collection and 10 mL of blood samples were collected by qualified staff from each participant. Saliva and serum were then stored at -80 °C until analysis.

Treatment:

Other: Saliva sampling

Amino Acid Analysis

Description:

Quantitative analysis of amino acids in saliva was carried out using liquid chromatography-tandem mass spectrometry (LC-MS/MS) with a Thermo Scientific TSQ Quantum Access MAX system (Thermo Scientific, Schaumburg, IL, USA).

Treatment:

Other: Amino Acid Analysis