Fructose-induced Intestinal de Novo Lipogenesis (FIDNL)

University of Lausanne (UNIL)

Status: Completed

Conditions: Obesity

Treatments: Other: fat+protein meal; Other: Fat+protein+13C fructose+glucose meal; Other: Fat + protein + 13C fructose meal; Other: 13C Fat + whey protein + glucose+ glucose meal; Other: 13C Fat + whey protein

Study type: Observational

Funder types: Other

Identifiers

NCT01792089

193/11

Details and patient eligibility

About

8 healthy volunteers, 8 pre-bypass obese subjects, 8 subjects between 12-18 months post-bypass, and 8 subjects matched to the post-bypass patients will be studied in two sub-studies Sub-study 1) after ingestion of two or three different of the following test-meals: A)cream and whey protein B)cream, whey protein and fructose + 13C6 fructose C)test meal with cream, whey protein, fructose and glucose + 13C6 fructose Their intestinal de novo lipogenesis will be estimated by measuring 13C-palmitate on triglyceride-rich lipoprotein particles associated with apoB48 in blood Sub-study 2) after ingestion of two or three different of the following test-meals: A)cream labelled with 13C6 palmitic acid and whey protein B))cream labelled with 13C6 palmitic acid, whey protein, fructose and glucose

Their intestinal de novo lipogenesis will be estimated by measuring 13C-palmitate on triglyceride-rich lipoprotein particles associated with apoB48 in blood in sub-study 1

Their exogenous lipid absorption kinetics will be assessed by measuring 13C palmitate on triglyceride-rich lipoprotein particles associated with apoB48 in sub-study 2

Enrollment: 24 patients

Sex: All

Ages: 25 to 50 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• age 18-50 years
• sex male or female
• less than 2 kg body weight change over the past 3 months subjects

Exclusion criteria

• antidiabetic and hypolipemic drugs
• alcohol consumption >20g/day
• severe eating disorders
• severe psychological problems
• vegetarian diet or other specific diet
• consumption of illicit substances
• pregnancy
• weight gain or weight loss > 1 kg in the last 3 months

Trial design

24 participants in 3 patient groups

non-obese healthy subjects

Description:

subjects with BMI<25 and no known disease

Treatment:

Other: 13C Fat + whey protein

Other: 13C Fat + whey protein + glucose+ glucose meal

Other: Fat + protein + 13C fructose meal

Other: Fat+protein+13C fructose+glucose meal

Other: fat+protein meal

post-gastric bypass

Description:

post-obese subjects 12-48 months after Roux-en-Y gastric bypass

Treatment:

Other: 13C Fat + whey protein + glucose+ glucose meal

Other: Fat + protein + 13C fructose meal

Other: fat+protein meal

matched control subjects

Description:

Nonobese, healthy subjects of similar age, weight, and gender ratio, than post-bypass subjects

Treatment:

Other: 13C Fat + whey protein + glucose+ glucose meal

Other: Fat + protein + 13C fructose meal

Other: fat+protein meal