Gasdermin-D and Pannexin-1 in Pregnancy

Haseki Training and Research Hospital

Status: Completed

Conditions: Maternal Obesity; Pregnancy Complications

Treatments: Diagnostic Test: Biomarker measurement of pannexin-1 and gasdermin-D

Study type: Interventional

Funder types: Other

Identifiers

NCT05475951

95932

Details and patient eligibility

About

One -third of fertile women around the world are overweight or obese. This means increasing risk for both the mother and the child. Fat tissue is a factor in gestational DM development and the increase in material inflammation and oxidative stress. According to new knowledge, it is an important need to examine molecules that are not handled in new and human research in these mechanisms in fat and placenta tissues in obesity. For this purpose, the examination of the expression of gasdermin-D and pannex-1 proteins, which are shown to be produced in the cells of both tissues, is worth investigating in human fat tissue and placenta. Gasdermins and pannexins are proteins intersecting by interacting in cellular functions.

Gasdermins cause piroptosis, a type of litic proinflammatory cell death. Pannexin-1 plays in various cellular functions, including inflammation. These are examined in a small number of in vitro studies in material fat tissue and placenta, and the design of this study in people whose applications are applied is original in humans.

The status of the expressions of the gasdermin-D and pannexin-1 proteins, which will be examined for the first time in obese pregnant women's fat and placental tissues, are the subject of this research with each other and their relationship with other maternal and neonatal data.

Enrollment: 79 patients

Sex: Female

Ages: 20 to 35 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Being in the 20-35 age range
• Term pregnancy (37-41 weeks)
• Healthy pregnancy follow-up
• Planned cesarean section
• Having no complication in cesarean delivery
• Being a placenta with front or rear walls

Exclusion criteria

• Intrauterine surgical procedure
• Presence of prenatal placental structural disorder
• Multiple pregnancies

Trial design

Primary purpose: Basic Science

Allocation: Non-Randomized

Interventional model: Parallel Assignment

Masking: None (Open label)

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79 participants in 3 patient groups

Normal Weight Pregnant Women

Experimental group

Description:

The women who had term pregnancy and had normal BMI.

Treatment:

Diagnostic Test: Biomarker measurement of pannexin-1 and gasdermin-D

Overweight Pregnant Women

Experimental group

Description:

The women who had term pregnancy and had BMI values between 25-30.

Treatment:

Diagnostic Test: Biomarker measurement of pannexin-1 and gasdermin-D

Obese Pregnant Women

Experimental group

Description:

The women who had term pregnancy, and had BMI values more than 30.

Treatment:

Diagnostic Test: Biomarker measurement of pannexin-1 and gasdermin-D