GCF miRNA-223 , -214 Levels in Smokers and Nonsmokers Periodontitis Patients

Fayoum University

Status: Completed

Conditions: Periodontitis, Adult

Treatments: Diagnostic Test: miRNA-223 & -214 detection

Study type: Observational

Funder types: Other

Identifiers

NCT06064799

REC-FDBSU/05012023-04/AM

Details and patient eligibility

About

Objective: Periodontitis is a multifactorial disease that affects a wide range of populations. However, its pathogenesis remains unclear. miRNAs are now considered potential diagnostic markers for many inflammatory diseases. Thus, the aim of this study was to assess the expression of microRNA-223(miRNA-223) and microRNA-214 (miRNA-214) in gingival crevicular fluid (GCF) of smoker and nonsmoker with periodontitis.

Materials and Methods: We conducted a prospective study among 42 participants: 14 healthy controls, 14 nonsmoker periodontitis participants, and 14 smoker with periodontitis. Eligibility criteria for inclusion were consecutive adults, aged 20-60 years, with stage III periodontitis grade B/C and no systemic diseases. All consenting participants had gingival crevicular fluid samples collected after diagnosis to assess miRNA-214 and -223 by quantitative real-time polymerase chain reaction assay.

Enrollment: 42 patients

Sex: All

Ages: 20 to 60 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• both genders
• age from 20 to 60 years
• no more than 4 teeth lost due to periodontitis
• systemically free participants

Exclusion criteria

• Pregnant or lactating female
• participants with a history of an inflammatory or immunological disease
• history of any current medication

Trial design

42 participants in 3 patient groups

Group I

Description:

14 healthy volunteers

Treatment:

Diagnostic Test: miRNA-223 & -214 detection

Group II

Description:

14 periodontitis participants with stage III grade B Periodontitis

Treatment:

Diagnostic Test: miRNA-223 & -214 detection

Group III

Description:

14 periodontitis participants with stage III grade C Periodontitis

Treatment:

Diagnostic Test: miRNA-223 & -214 detection