Generation of Haploid Stem Cells From Human Germ Cells

Rambam Health Care Campus

Status: Unknown

Conditions: Induced Pluripotent Stem Cells

Treatments: Other: generation of stem cells

Study type: Observational

Funder types: Other

Identifiers

NCT01454765

rmb-maza0001-ctil

Details and patient eligibility

About

Human cells are diploid (contain a double set of chromosomes). The diploid genome is complex and therefore limits genetic research approaches in biomedical models. To overcome this problem experimental generation of haploid stem cells has been done in animals. The investigators aim at generating haploid stem cells from human germ cells that are a useful tool in genetic research and screening.

Full description

Human cells are diploid (contain a double set of chromosomes). The diploid genome is complex and therefore limits genetic research approaches in biomedical models. To overcome this problem experimental generation of haploid stem cells has been done in animal models. haploid cells are easier for genetic manipulations, such as knock-in and knock-out of genes, and therefore should make a better experimental model. The investigators aim at generating haploid stem cells from human haploid germ cells.

Enrollment: 10 estimated patients

Sex: Male

Ages: 18 to 50 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

1. males over the age of 18 and under 50
2. no known chronic illness
3. not taking any chronic drugs

Exclusion criteria

1. females
2. males under 18 or over 50
3. any known chronic illness
4. taking chronic medications

Trial design

10 participants in 1 patient group

Sperm sample from healthy volunteers

Treatment:

Other: generation of stem cells