Genetics of Ischemic Cardiomyopathy

Cairo University (CU)

Status: Completed

Conditions: Ischemic Cardiomyopathy

Treatments: Procedure: Cardiac biopsy, blood sampling; Genetic: quantitative polymerase chain reaction(QPCR) for four genes

Study type: Observational

Funder types: Other

Identifiers

NCT05508269

RHFCS1

Details and patient eligibility

About

Background:Ischemic heart disease is one of the heaviest health-related burdens worldwide.We aimed to identify the common hub mRNA and pathways that are involved in pathological progression of ischemic cardiomyopathy. Methods: To explore potential differentially expressed genes (DEGs) of all ischemic heart disease stages, we used chipster and GEO2R tools to analyze of retrieved eight high throughput RNA datasets obtained from GEO database. Gene Ontology functional annotation and Pathways enrichment analyses were used to obtain the common functional enriched DEGs which were visualized in protein-protein interactions (PPI) network to explore the hub mRNA according to the interaction scores. Validation qRT-PCR was carried out for blood and cardiac biopsies compared with controls to validate the determined four hub mRNAs and subsequently reviewed inside comprehensive published meta-analysis database. The validated mRNAs were visualized in two interaction modules. Finally screening of approved drugs was applied.

Enrollment: 26 patients

Sex: All

Ages: 18 to 80 years old

Volunteers: Accepts Healthy Volunteers

Inclusion and exclusion criteria

Inclusion criteria The enrolled patients aged 20-80 years with stable coronary artery disease undergoing elective PCI or patients with unstable angina or myocardial infarction undergoing rescue PCI and admitted to the cardiology hospital (Asyut University, Asyut, Egypt) were recruited in this study.

Exclusion criteria Patients with dilated cardiomyopathy were execluded from this study.

Trial design

26 participants in 4 patient groups

Control subjects

Description:

control cases who were diagonosed as healthy subjects after diagnostic catheterization

Treatment:

Procedure: Cardiac biopsy, blood sampling

Genetic: quantitative polymerase chain reaction(QPCR) for four genes

Stable coronary artery disease

Description:

patients with stable coronary artery disease undergoing elective PCI who were diagnozed with coronary lesion/s and underwent stent implantation without previous history of myocardial infarction

Treatment:

Procedure: Cardiac biopsy, blood sampling

Genetic: quantitative polymerase chain reaction(QPCR) for four genes

Myocardial infarction patients

Description:

patients with history of myocardial infarction or with acute myocardial infarction undergoing primary PCI

Treatment:

Procedure: Cardiac biopsy, blood sampling

Genetic: quantitative polymerase chain reaction(QPCR) for four genes

Ischaemic cardiomyopathy patients

Description:

patients with ischaemic cardiomyopathy diagnosed by ejection fraction less than 35% with history of coronary artery disease or myocardial infarction or those with acute myocardial infarction with reduced ejection fraction.

Treatment:

Procedure: Cardiac biopsy, blood sampling

Genetic: quantitative polymerase chain reaction(QPCR) for four genes