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Genetics of Peripheral Artery Genomics

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Duke University

Status

Completed

Conditions

Peripheral Artery Disease

Study type

Observational

Funder types

Other

Identifiers

NCT00615121
Pro00008270
4814-07 (Other Identifier)

Details and patient eligibility

About

Peripheral artery disease is a disease that contributes to significant morbidity and mortality of millions of Americans yearly. Very little is known about the pathophysiology of atherosclerosis in peripheral artery disease. We plan to collect peripheral arteries and muscle tissue from patients undergoing amputation for end stage peripheral arterial occlusive disease. By extracting the RNA from these arteries and tissues and comparing them with RNA expression from normal arteries, we hope to have a better understanding of the pathophysiology of atherosclerosis in this setting. We aim to prove the hypothesis that a novel gene expression pattern can be discovered by the successful extraction of RNA from plaques from human peripheral arteries.

Full description

Patients scheduled to undergo lower extremity amputation will be consented for extraction of plaque from the carotid artery or arterial tissue from the amputated limbs. The surgeon will perform the amputation, then the harvest team will collect arterial tissue from amputated limbs. These samples will be immersed in RNALater solution to preserve the integrity of the RNA. Tissues will stored at -80oC in the RNALater until the RNA is later extracted using a standardized protocol. The RNA will be examined for quality using an Agilent bioanalyzer, and will then be processed on Affymetrix U133A chips for gene expression data. The data will be analyzed with the MATLAB software package.

Enrollment

122 patients

Sex

All

Volunteers

Accepts Healthy Volunteers

Inclusion criteria

  • Patients undergoing amputations for peripheral artery disease
  • Patients undergoing free fibular transfer

Exclusion criteria

  • those that are not able to consent to the study

Trial design

122 participants in 2 patient groups

arteries from PAD patients
Description:
peripheral arteries from patients undergoing amputation for end stage peripheral arterial occlusive disease
arteries from Free Fib transfers
Description:
peripheral arteries from patients without evidence of peripheral arterial occlusive disease

Trial contacts and locations

1

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Data sourced from clinicaltrials.gov

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