Genomic DNA in Human Blastocoele Fluid

Cervesi Hospital, Cattolica, Italy

Status: Unknown

Conditions: Single Gene Desease; Chromosomal Aneuploidies

Treatments: Other: blastocoele aspiration

Study type: Observational

Funder types: Other

Identifiers

NCT01780415

NewEra21972

Details and patient eligibility

About

The objective will focus on the characterization of DNA fragments present in Blastocoele fluid, and the evaluation of these fragments as potential target for Preimplantation Genetic Diagnosis. To reach this goal, real-time PCR, Whole Genome Amplification techniques and -subsequently- Next Generation Sequencing and aCGH approaches will be used.

Enrollment: 35 patients

Sex: Female

Ages: 18 to 40 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• IVF patient with supernumerary embryos to vitrify at blastocyst stage

Exclusion criteria

• IVF patient without blastocyst formation

Trial design

35 participants in 1 patient group

Bastovit

Description:

all the patients that have supernumerary embryos to vitrify at blastocyst stage

Treatment:

Other: blastocoele aspiration