Genomics in Infection and Sepsis to Predict Organ Dysfunction and Outcomes in Sepsis

The Chinese University of Hong Kong

Status: Unknown

Conditions: Infection; Pneumonia; Sepsis

Study type: Observational

Funder types: Other

Identifiers

NCT04199962

2019.372

Details and patient eligibility

About

This is a prospective cohort study using gene expression to study patients with infection and sepsis from pneumonia.

Full description

This is a prospective cohort study using single cell transcriptomic profiling and plasma DNA tissue mapping on patients with pneumonia with or without sepsis. The major application of the investigator's study would be the discovery of gene expressions in different leucocytes and plasma DNA associated with each type of organ dysfunction in sepsis. These include cardiovascular, respiratory, hepatic, renal, neurological and haematological dysfunction. This would help prediction, diagnosis and development of therapies to treat sepsis. Leucocyte single cell transcriptome and plasma DNA tissue mapping may addresses the limitations of current evidence in 3 ways: (1) differentiate patients with uncomplicated pneumonia versus pneumonia with associated sepsis, (2) correlation with types and severity of organ dysfunction and (3) identifying molecular phenotypes of sepsis.

Enrollment: 120 estimated patients

Sex: All

Ages: 18+ years old

Volunteers: No Healthy Volunteers

Inclusion criteria

All of the following:

• newly admitted adult patients (≥ 18 years old)
• suspected community acquired pneumonia (CAP)
• compatible history of either sputum or cough or fever or rigors within 1 week
• chest X-ray infiltrates

Exclusion criteria

Any of the following:

• chest symptoms not solely accounted by pneumonia (cardiac failure, non cardiogenic pulmonary oedema, suspected pulmonary embolism, suspected secondary acute respiratory distress syndrome)
• immunosuppression
• current malignancy
• blood samples for gene expression could not be taken within 24 hours of admission
• prisoner/cogni tive impairment
• blood transfusion within 1 month
• hospitalization within 1 month

Trial design

120 participants in 2 patient groups

pneumonia without sepsis

Description:

adult patients with community acquired pneumonia change in SOFA score \<2 (other than respiratory component)

pneumonia with sepsis

Description:

adult patients with community acquired pneumonia change in SOFA score greater or equal to 2 (other than respiratory component)