Glioma Microenvironment an Exploratory Study

Hospital Sirio-Libanes

Status: Active, not recruiting

Conditions: Glioma of Brain

Treatments: Procedure: Tumor resection

Study type: Observational

Funder types: Other

Identifiers

NCT03189420

CAAE 58310716.6.0000.5461

Details and patient eligibility

About

Diffuse glioms are primary brain tumors characterized by infiltrative growth and high heterogeneity, which render the disease mostly incurable. Advances in genetic analysis revealed that molecular and epigenetic alterations predict patients´s overall survival and clinical outcome. However, glioma tumorigenicity is not exclusively caused by its genetic alterations. The crosstalk between tumor cells and the surrounding microenvironment plays a crucial role in modulating glioma growth and aggressiveness. In this sense, to understand the tumor microenvironment would elucidate potential treatment alternatives. The focus will be to evaluate myeloid cells and cytokines levels.

Full description

The proposal is to study gene expression and early epigenetic changes in myeloid cells from brain tumors and co-culture experiments. Tough RNA-seq, ATAC-seg and subsequent analysis to monitor the differential gene expression through different time points followed by stimuli. The study will compare the obtained results with sequencing data from microglia of full-fledged human tumor samples (glioblastoma and low grade gliomas). Additionally it will evaluated the immunologic characteristic of the tumor through cytokines levels analysis (TNF-α, IL-4, IL-6, IL-8, IL-10, IL-13, IFN-γ, TGF-β e IL-1ra).

Enrollment: 30 estimated patients

Sex: All

Ages: 18 to 65 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• 18 - 65 years old with brain tumor
• Patients that will be submitted to brain tumor ressection

Exclusion criteria

• Chronic neurodegenerative and inflamatory diseases

Trial design

30 participants in 2 patient groups

Glioblastoma

Description:

Samples of brain tumor diagnosed as glioblastoma

Treatment:

Procedure: Tumor resection

Low grade glioma

Description:

Samples of brain tumor diagnosed as low grade glioma

Treatment:

Procedure: Tumor resection